Biomod/2012/TeamSendai/Idea

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Team Sendai Top

Contents

Project

The best way to that carried on our project is to divide it experimentally in different parts. Lucky for us that we have a large number of people in our team (being most of us freshman!). So we decided to separate our project into several parts and do experiments in parallel. Our experiments are divided into three parts; GATE part, PORTER part, and MEMBRANE part.

GATE part is the group making the CELL-GATE nanostructure.

PORTER part is the group making the transporting mechanism of the target into the GATE.

MEMBRANE part is the group making liposome by using lipid.

To separate our project and finally mix the parts altogether, we aim to gain our achievement. And we have also established SIMULATION group that theoretically verifies each structure.

Project GATE

Goal

GATE is the gatekeeper that allows only the target to enter the cell. Electric repulsions from the negative charge of the DNA backbone inhibit NOT desired DNA from entering GATE.


Project PORTER

Goal

PORTER is in charge of the active transporting of the target into GATE. The first Porter is likely to be outside GATE because of its electric repulsion. Furthermore, the first Porter catches the target DNA and pull it inside the GATE by hybridizing with it. Inner Porters that have higher affinity than the first Porter pull the target inside GATE step by step.


Project MEMBRANE

Goal

Picture taken from "Molecular biology of the CELL" (Bruce Alberts et al.)
Picture taken from "Molecular biology of the CELL" (Bruce Alberts et al.)

As active transporter, "CELL-GATE" should work in a cell membrane. Thus, a implementation module for inserting it to membranes was designed. DNA sequences with a hydrophobic molecule (cholesterol) are situated outside and around GATE. We used liposome (artificial lipid vesicle) as a model cell membrane.


Application in future

Finally, this project aims to attach to a real cell and transport a substance to cell and from cell. Of course, this channel may have medical use. Also, it may be used for bringing some substances which are difficult to bring back from cell nowadays. In our project "CELL-GATE", we used liposome as a model of a cell membrane, but if we consider that the "CELL-GATE" is attached to liposome, it can be used as molecular robot. This robot may be used as a cleaning robot or medical sprinkling robot.

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