We want to make an artificial channel.If we make an artificial channel, it is desirable that the channel can carry selectively and actively only what we want to carry. Also, it is desirable that the channel can change its function and shape artificially. Think of these things, we think it is best to make this structure with DNA-origami. If we use DNA, the shape of the channel is changeable as we like. Also, the carry structure that through the channel is possible by using DNA’s complementarity, so we decided to use DNA to make the structure.
When we test the effect of the Cell-gate, we use liposome as a model of a cell membrane. We have to make liposome and confirm the effect of the Cell-gate. So finally, we will make molecular robot which is Cell-gate on liposome and transport the object inside or outside liposome. In short, our goal in this experiment is following.
Three experiment parts
But on experiment, it is not smart that proceeing our project in order. Luckily, large number of people in our team(and most of us are fresh!). So we decided to separate our project into several part and do experiment parallelly. Our experiment separates three parts; Gate part, Porter part, and Membrane part.
Gate part is the group making the Cell-gate itself.
Porter part is the group making the function to transport the target in the channel.</br>
Membrane part is the group making liposome by using lipid.
To separate our project and finally mix, we aim to gain our achievement.
And we also establish simmulation group that verifies each structure theoretically.
We will make the gate made of DNA origami! DNA origami is the way how to fold DNA and make structure investigated by Paul Rothemund.
So we decided to make the tube structure as the Gate using DNA origami. Consideration for the form of the Gate is written on Design page "Gate". In the Gate, "Porter" which transport the target is planted. So Gate can connect the inside and outside.
We can use the Gate as an injector or extractor.
The simulation that the targets actually enter in the Gate is here.
We considered about annealing situation of the Gate and did electrophoresis and AFM for observing the Gate.
Consideration for annealing situation and experiment results is here.
We thought to make DNA Porter which is function to transport the target in channel. One of the characteristic of DNA is to bind another DNA comprementary sequence to it.
If we design that the DNA binds the target more stable than former one, and if next DNA binds more stable than it…,
the target moves to most complementary sequence DNA.
We thought this characteristic of DNA can be utilized the power of channel.
This channel can make us transport the object selectively and actively independent of concentration gradient.
We deceided to make the Porter made of DNA.
We did electrophoresis to confirm working of DNA. Experiment method and result is here.
In this project, We make the model of cell membrane and aim that the channel penetrate it.
Because using cell membrane immediately is hard.
We make liposome by using lipid.
Liposome is the membrane made of lipid and utilize liposome as model of cell-membrane.
Consideration making situation of liposome is here.
And experiment method and result is here.
Application in future
Finally, this project aims to attach a channel to a real cell and transport a substance form inside to outside. Of corse, this channel can have medical use. Also, it can be used for bringing some substance which is difficult to bringback now from cells. In our project, we used liposome as a model of a cellular membrane, but if we consider the channel to be attached to liposome working as a molecular robots, then the robot can be used as a cleaning robot or medical injecting robot.