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Biomod/2012/TU Dresden/Nanosaurs/Project/Aptamer lock - Revision history
2013-05-25T16:11:03Z
Revision history for this page on the wiki
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http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=649018&oldid=prev
Karen Elda Viacava Romo at 20:04, 29 October 2012
2012-10-29T20:04:22Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Each lock is essentially composed of two complementary oligonuleotidic strands - an aptamer strand specific to PDGF and a strand complementary to it.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Each lock is essentially composed of two complementary oligonuleotidic strands - an aptamer strand specific to PDGF and a strand complementary to it.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><b><big>Aptamer strand: <code class="dna_blue"><del class="diffchange diffchange-inline">&nbsp;</del>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5'TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA3'</code></big></b></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><b><big>Aptamer strand: <code class="dna_blue">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5'TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA3'</code></big></b></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><b><big>Aptamer locking strand: <code class="dna_green">3'ATGAGTCCCGACACGTTCGTTAACACCAGGGTTACCCGACTCAT5'</code></b></big></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><b><big>Aptamer locking strand: <code class="dna_green">3'ATGAGTCCCGACACGTTCGTTAACACCAGGGTTACCCGACTCAT5'</code></b></big></p></div></td></tr>
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Karen Elda Viacava Romo
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648970&oldid=prev
Gilbert S. Canon at 16:33, 29 October 2012
2012-10-29T16:33:27Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align = "justify">PDGF was always used in a 10 times excess concentration than that of the lock. All the samples were incubated at room temperature for 24 Hrs.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align = "justify">PDGF was always used in a 10 times excess concentration than that of the lock. All the samples were incubated at room temperature for 24 Hrs.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> The results of this experiment are presented in Fig. 8. The results shown were obtained from averaging the measurements from three sets of experiments. The error bars show the standard deviation <del class="diffchange diffchange-inline">with </del>is reasonably low. Hence, the results were consistent over multiple experiments</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> The results of this experiment are presented in Fig. 8. The results shown were obtained from averaging the measurements from three sets of experiments. The error bars show the standard deviation <ins class="diffchange diffchange-inline">which </ins>is reasonably low. Hence, the results were consistent over multiple experiments</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p> From the results obtained, it was observed hat the blockers work better than PDGF at opening the lock hybrid. Blocker 1 was found to be more </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p> From the results obtained, it was observed hat the blockers work better than PDGF at opening the lock hybrid. Blocker 1 was found to be more </div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648969&oldid=prev
Gilbert S. Canon at 16:15, 29 October 2012
2012-10-29T16:15:37Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center" align = "center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center" align = "center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p align = "center"><del class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</del>Fig. 3 Legend<p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p align = "center">Fig. 3 Legend<p></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648968&oldid=prev
Gilbert S. Canon at 16:14, 29 October 2012
2012-10-29T16:14:40Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center" align = "center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center" align = "center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">align = "center"</ins>>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648967&oldid=prev
Gilbert S. Canon at 16:13, 29 October 2012
2012-10-29T16:13:21Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="img_center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="img_center<ins class="diffchange diffchange-inline">" align = "center</ins>"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648963&oldid=prev
Gilbert S. Canon at 15:42, 29 October 2012
2012-10-29T15:42:11Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Samples</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Samples<ins class="diffchange diffchange-inline">:</ins></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Lock</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Lock</li></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648962&oldid=prev
Gilbert S. Canon at 15:39, 29 October 2012
2012-10-29T15:39:35Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Unspecific ds-DNA, one strand labeled with Cy3</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Unspecific ds-DNA, one strand labeled with Cy3</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Unspecific short ds-DNA, one strand labeled with Cy3; PDGF</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Unspecific short ds-DNA, one strand labeled with Cy3; PDGF</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li>Lock<del class="diffchange diffchange-inline">,</del>aptamer strand without BHQ, aptamer locking strand with Cy3; blocker 1 and blocker 2</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li>Lock <ins class="diffchange diffchange-inline">- </ins>aptamer strand without BHQ, aptamer locking strand with Cy3; blocker 1 and blocker 2</li></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648961&oldid=prev
Gilbert S. Canon at 15:29, 29 October 2012
2012-10-29T15:29:57Z
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648960&oldid=prev
Gilbert S. Canon at 15:29, 29 October 2012
2012-10-29T15:29:32Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</ins>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td></tr>
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Gilbert S. Canon
http://openwetware.org/index.php?title=Biomod/2012/TU_Dresden/Nanosaurs/Project/Aptamer_lock&diff=648959&oldid=prev
Gilbert S. Canon at 15:29, 29 October 2012
2012-10-29T15:29:04Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:29, 29 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="img_center"><a rel="lightbox[aptamer_leg]" title="Legend" href="http://openwetware.org/images/e/ed/BM12_Nanosaurs_Aptamer_legend_800.jpg"><img src="http://openwetware.org/images/f/fc/BM12_Nanosaurs_Aptamer_legend_500.jpg"></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>&nbsp&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>&nbsp<ins class="diffchange diffchange-inline">;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</ins>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 3 Legend<p></div></td></tr>
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Gilbert S. Canon