Biomod/2011/UTAustin/Hook'em Hybridizers:Results: Difference between revisions

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<span style="font-size:40px; font-family: Arial; font-weight:bold; color:#CC5500; vertical-align: middle;">The Hook'em Hybridizers</span>
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===AND Gate Kinetics===
To experimentally prove that our counter functions correctly, we needed to test the AND gate to ensure it works as intended. In the graph below we added A2andX together with our input -X to determine if leakage would occur. Though there was some, as seen by comparison with two controls, we believe it is not significant. After forty minutes, we added the first input to the AND gate, A1-A2 and as seen, immediately begins to produce signal just as intended. This allows us to verify that our AND gate is a viable part of our design.
[[Image:Andgatekinetics.png|620px]]
===Gels===
===Gels===
Shown below are fluorescence images of acrylamide gels that have been stained with SYBR Gold 1000X fluorescent dye. The measurements were taken at 450nm (blue).
Shown below are fluorescence images of acrylamide gels that have been stained with SYBR Gold 1000X fluorescent dye. The measurements were taken at 450nm (blue).
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====G_X, G_A, G_A1, T_A1====
====G_X, G_A, G_A1, T_A1====
This gel has three gates and a threshold. The color washes out at relatively high concentrations of DNA meaning the white bulges in the gel are where the majority of DNA lie, which correspond to the correct length of nt.
This gel has three gates and a threshold. The color washes out at relatively high concentrations of DNA meaning the white bulges in the gel are where the majority of DNA lie, which correspond to the correct length of nt.
                          G_X             G_A             G_A1             T_A1.
                  G_X         G_A         G_A1         T_A1.
   
   
[[Image:G X,A,A1 T A1.png|620px]]
[[Image:G X,A,A1 T A1.png|620px]]
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====A2andX====
====A2andX====
This gel shows our synthesized AND gate. Each lane represents a different possible combination of components. The left two are the top strands for the gate itself while the right four are the different combinations the top strands can make with the gate.
This gel shows our synthesized AND gate. Each lane represents a different possible combination of components. The left two are the top strands for the gate itself while the right four are the different combinations the top strands can make with the gate.
                                A2-    X-B  A2andX:A2-,X-B     A2andX:X-B
                      A2-    X-B  A2andX:A2-,X-B   A2andX:X-B
                                                    A2andX:A2-,X-B     A2andX:A2-
                                        A2andX:A2-,X-B   A2andX:A2-
 
[[Image:andgate-noskew2.png|620px]]
 
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[[Image:A2andX.png|620px]]
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Latest revision as of 09:12, 3 November 2011

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Results

AND Gate Kinetics

To experimentally prove that our counter functions correctly, we needed to test the AND gate to ensure it works as intended. In the graph below we added A2andX together with our input -X to determine if leakage would occur. Though there was some, as seen by comparison with two controls, we believe it is not significant. After forty minutes, we added the first input to the AND gate, A1-A2 and as seen, immediately begins to produce signal just as intended. This allows us to verify that our AND gate is a viable part of our design.

Gels

Shown below are fluorescence images of acrylamide gels that have been stained with SYBR Gold 1000X fluorescent dye. The measurements were taken at 450nm (blue).

X-

The fuel strand for the first gate (G_X) as well as the AND gate.

X- CUT

The "cut" image of the fuel strand verifying the proper location of collection.

G_X, G_A, G_A1, T_A1

This gel has three gates and a threshold. The color washes out at relatively high concentrations of DNA meaning the white bulges in the gel are where the majority of DNA lie, which correspond to the correct length of nt. 

                  G_X          G_A         G_A1          T_A1.

A2andX

This gel shows our synthesized AND gate. Each lane represents a different possible combination of components. The left two are the top strands for the gate itself while the right four are the different combinations the top strands can make with the gate. 

                      A2-    X-B  A2andX:A2-,X-B   A2andX:X-B
                                       A2andX:A2-,X-B   A2andX:A2-

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