Biomod/2011/UTAustin/Hook'em Hybridizers:Results: Difference between revisions
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Revision as of 16:45, 1 November 2011
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Results
Gels
Shown below are fluorescence images of acrylamide gels that have been stained with SYBR Gold 1000X fluorescent dye. The measurements were taken at 450nm (blue).
X-
The fuel strand for the first gate (G_X) as well as the AND gate.
X- CUT
The "cut" image of the fuel strand verifying the proper location of collection.
G_X, G_A, G_A1, T_A1
This gel has three gates and a threshold. The color washes out at relatively high concentrations of DNA meaning the white bulges in the gel are where the majority of DNA lie, which correspond to the correct length of nt.
G_X G_A G_A1 T_A1.
A2andX
This gel shows our synthesized AND gate. Each lane represents a different possible combination of components. The left two are the top strands for the gate itself while the right four are the different combinations the top strands can make with the gate.
A2- X-B A2andX:A2-,X-B A2andX:X-B
A2andX:A2-,X-B A2andX:A2-
