Biomod/2011/UTAustin/Hook'em Hybridizers: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(90 intermediate revisions by 5 users not shown)
Line 1: Line 1:
[[Image:Placeholder.png|150px]]
<html><head>
<div style="padding: 10px; color: #FF6633; background-color: #000; width: 730px">
<style type="text/css">
#outerbox{
//background-image:url( 'http://openwetware.org/images/7/75/Utbiomodbg1.png' );
//background-repeat:repeat-x;
background-image: linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);
background-image: -o-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);
background-image: -moz-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);
background-image: -webkit-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);
background-image: -ms-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);
 
background-image: -webkit-gradient(
linear,
left bottom,
left top,
color-stop(0.09, rgb(0,0,0)),
color-stop(0.55, rgb(204,85,0))
);
}
 
.navbox {
position: relative;
float: left;
}
 
ul#nav {
    list-style: none;
    display: block;
    width: 200px;
    left: -29px;
    top: -20px;
    position: relative;
    padding: 60px 0 60px 0;
    width: 200px;
    background: url( 'http://openwetware.org/images/6/6f/Shad2.png' ) no-repeat;
    background-size: 50% 100%;
}
 
li {
margin: 5px 0 0 0;
}
 
ul#nav a:hover {
    text-decoration: none;
}
 
ul#nav li span {
    -webkit-transition: all 0.3s ease-out;
    -moz-transition: all 0.3s ease-out;
    -o-transition: all 0.3s ease-out;
    background: #E07020 url( 'http://openwetware.org/images/4/46/Border.png' ) no-repeat;
    color: #174867;
    padding: 7px 15px 7px 15px;
    border-top-right-radius: 10px;
    border-bottom-right-radius: 10px;
    width: 100px;
    display: block;
    text-decoration: none;
    box-shadow: 2px 2px 4px #BB4400;
}
 
ul#nav li span:hover {
    background: #F08030 url( 'http://openwetware.org/images/4/46/Border.png' ) no-repeat;
    color: #67a5cd;
    padding: 7px 15px 7px 30px;
    display: block;
}
 
</style>
</head>
</html>
 
<div id="outerbox" style="padding-top:10px; padding-bottom:10px;">
 
<div id="innerbox" style="width: 820px; padding: 15px; margin: auto; background-color:#000000; border-radius: 5px">
 
<div id="header" style="background-color:#000000;">
<center>
<center>
[[Image:UTBiomodBanner2.png|780px]]
</center>
</div>
<div style="padding: 10px; color: #FFFFFF; background-color: #CC5500; width: 130px; height: 1025px; float: left;">
<div id="navbox">
<ul id="nav">
<li>
[[Biomod/2011/UTAustin/Hook'em_Hybridizers | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Home'''</span>  ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em_Hybridizers:Rationale | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Rationale'''</span> ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em Hybridizers:Simulation | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Simulation'''</span> ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em Hybridizers:Methods | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Methods'''</span> ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em Hybridizers:Results | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Results'''</span> ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em Hybridizers:Tools | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''Tools'''</span> ]]
</li>
<li>
[[Biomod/2011/UTAustin/Hook'em Hybridizers:People | <span style='font-size:16px; font-family: "trebuchet ms"; font-weight:bold; color:#FFFFFF; vertical-align: middle; text-decoration: none;'>'''People'''</span> ]]
</li>
</ul>
</div>
</div>
<div style="width: 650px; float: left; padding: 0px; background-color: #000000;">
<div style="width: 320px; text-align: left; float: left; margin-left: 5px">
<div style="height: 30px; background-color: #3F70C3; padding: 10px 10px 10px;">
<span style="font-size: 20px; font-family: Verdana; color:#FFFFFF; vertical-align: middle;">Objective</span>
</div>
<div style="padding: 10px; background-color:#000000; color: #FFFFFF; margin-top: 5px; height: 345px;">
<center>'''Counting Events with a DNA Processor'''</center>
A major goal in nanotechnology is the find useful forms of DNA computation. This broad definition includes amplifiers, logic gates, oscillators, structural manipulations, and many more forms of controlled responses. We believe an enzyme-free, DNA toehold exchange catalyzed system that preforms the computation of “counting” can be useful in various fields of biotechnology. Counting, by our definition, relies on two main principles. The first is the input that is being counted must be the same with each pulse and the second is the output of the counter must be independent of the input quantity or concentration. That is, regardless of the amount of input used, the counter must only reach completion after the specified number of counts, or pulses.
</div>
</div>


[[The Hook'em Hybridizers | <font face="trebuchet ms" style="color:#FF6633"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<div style="height: 30px; width: 320px; text-align: left; float: right; margin-left: 5px;">
[[The Hook'em Hybridizers:Rationale | <font face="trebuchet ms" style="color:#FF6633"> '''Rationale''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
[[The Hook'em Hybridizers:Stimulation | <font face="trebuchet ms" style="color:#FF6633"> '''Stimulation''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<div style="background-color: #3F70C3; padding: 10px 10px 10px;">
[[The Hook'em Hybridizers:Methods | <font face="trebuchet ms" style="color:#FF6633"> '''Methods''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<span style="font-size: 20px; font-family: Verdana; color:#FFFFFF; vertical-align: middle;">The essence of a counter</span>
[[The Hook'em Hybridizers:Results | <font face="trebuchet ms" style="color:#FF6633"> '''Results''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</div>
[[The Hook'em Hybridizers:People | <font face="trebuchet ms" style="color:#FF6633"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[The Hook'em Hybridizers:Contact | <font face="trebuchet ms" style="color:#FF6633"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</center>
</div><br>


<div style="padding: 10px; background-color:#000000; margin-top: 5px; height: 345px;">
<center>[[Image:Counteressence.png|300px]]</center>
</div>


*Team name: The Hook'em Hybridizers
</div>
*Institution: The University of Texas at Austin


*Team Members
<div style="width: 645px; text-align: left; float: left; margin-left: 5px">
**Nadia Menouar
**Ryan Truby
**[[User:Ben_Braun|Ben Braun]]
**[[User:Victor J. Kronberg|Victor Kronberg]]
**[[User:Jeremy R. McLain|Jeremy McLain]]
**[[User:Nagina Naz|Nagina Naz]]
**Marguerite West-Driga
**Dan Winkler
**[[User:Michael Akilian|Michael Akilian]]


*Postdoc Mentors
<div style="padding: 10px; background-color:#000000; margin-top: 5px; height: 600px;">
**Dr. Peter Allen
**[[User:Xi_Chen|Dr. Xi Chen]]


*Faculty mentors
<html><object style="height: 390px; width: 640px"><param name="movie" value="http://www.youtube.com/v/hFEjbkRfkL0?version=3&feature=player_detailpage"><param name="allowFullScreen" value="true"><param name="allowScriptAccess" value="always"><embed src="http://www.youtube.com/v/hFEjbkRfkL0?version=3&feature=player_detailpage" type="application/x-shockwave-flash" allowfullscreen="true" allowScriptAccess="always" width="640" height="360"></object></html>
**[http://ellingtonlab.org/ Dr. Andrew Ellington]


* Common materials
</div>
** 1x TBE
*** Component
*** Recipe
*** Location


** 10% APS
</div>
*** Recipe
*** Location


* Lab techniques
<div style="width: 600px; clear: both"></div>
** How to make denaturing polyacrylamide gels


</div>


<div style="width: 800px; clear: both"></div>


</div>


----
</div>
The Hook'em Hybridizers front page was last edited by {{REVISIONUSER}}.

Latest revision as of 23:37, 2 November 2011

<html><head> <style type="text/css">

  1. outerbox{

//background-image:url( 'http://openwetware.org/images/7/75/Utbiomodbg1.png' ); //background-repeat:repeat-x; background-image: linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%); background-image: -o-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%); background-image: -moz-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%); background-image: -webkit-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%); background-image: -ms-linear-gradient(bottom, rgb(0,0,0) 9%, rgb(204,85,0) 55%);

background-image: -webkit-gradient( linear, left bottom, left top, color-stop(0.09, rgb(0,0,0)), color-stop(0.55, rgb(204,85,0)) ); }

.navbox { position: relative; float: left; }

ul#nav { 

   list-style: none;
   display: block;
   width: 200px;
   left: -29px;
   top: -20px;
   position: relative;
   padding: 60px 0 60px 0;
   width: 200px;
   background: url( 'http://openwetware.org/images/6/6f/Shad2.png' ) no-repeat;
   background-size: 50% 100%;

}

li { margin: 5px 0 0 0; }

ul#nav a:hover { 

    text-decoration: none;

}

ul#nav li span { 

   -webkit-transition: all 0.3s ease-out;
   -moz-transition: all 0.3s ease-out;
   -o-transition: all 0.3s ease-out;
   background: #E07020 url( 'http://openwetware.org/images/4/46/Border.png' ) no-repeat;
   color: #174867;
   padding: 7px 15px 7px 15px;
   border-top-right-radius: 10px;
   border-bottom-right-radius: 10px;
   width: 100px;
   display: block;
   text-decoration: none;
   box-shadow: 2px 2px 4px #BB4400;

}

ul#nav li span:hover { 

   background: #F08030 url( 'http://openwetware.org/images/4/46/Border.png' ) no-repeat;
   color: #67a5cd;
   padding: 7px 15px 7px 30px;
   display: block;

}

</style> </head> </html>

Objective

Counting Events with a DNA Processor

A major goal in nanotechnology is the find useful forms of DNA computation. This broad definition includes amplifiers, logic gates, oscillators, structural manipulations, and many more forms of controlled responses. We believe an enzyme-free, DNA toehold exchange catalyzed system that preforms the computation of “counting” can be useful in various fields of biotechnology. Counting, by our definition, relies on two main principles. The first is the input that is being counted must be the same with each pulse and the second is the output of the counter must be independent of the input quantity or concentration. That is, regardless of the amount of input used, the counter must only reach completion after the specified number of counts, or pulses.

The essence of a counter

<html><object style="height: 390px; width: 640px"><param name="movie" value="http://www.youtube.com/v/hFEjbkRfkL0?version=3&feature=player_detailpage"><param name="allowFullScreen" value="true"><param name="allowScriptAccess" value="always"><embed src="http://www.youtube.com/v/hFEjbkRfkL0?version=3&feature=player_detailpage" type="application/x-shockwave-flash" allowfullscreen="true" allowScriptAccess="always" width="640" height="360"></object></html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2011/UTAustin/Hook%27em_Hybridizers&oldid=563986"