Biomod/2011/Tianjin:Results: Difference between revisions
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==='''Fluorescence micrograph'''=== | ==='''Fluorescence micrograph'''=== | ||
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 6. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]] | [[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 6. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]] | ||
=='''Achievement and Prospect'''== | |||
===A “bottom-up” strategy to build up artificial cell surrogates=== | |||
*We successfully synthesized the actin-like cytoskeleton filaments in cell-free system and assembling components to create artificial cell surrogates. Bionanotechnology and microfluidics technology are also combined to artificially mimic the synthesis of sub-cellular structural unit which might contribute to recognizing, exploring and even creating life. | |||
===Brand-new system for study on modular organism=== | |||
*Normally the study of exogenous genes and proteins could be conducted in certain modular organism like E. coli and yeast. However, the research of structure and lethal components in modular organism sometimes comes to a dilemma, as we cannot find a better chassis, and the commonly-used methods such as deletion is not applicable any more. The cell-free system could serve as an optimum chassis which combines transcription and translation processes and avoid the limit of cell support on the expression of modular organism protein as well as some special noxious proteins. | |||
===Biological compatibility=== | |||
*It has been already discovered that CNTs (Carbon Nanotubes) are poisonous to many kinds of microorganisms. With the aggregation and spirally binding of filamentous cytoskeleton protein MreB on SWNTs (single-walled carbon nanotubes), their surface physical and chemical properties are enormously modified. With further characterization, this kind of SWNTs may become more compatible to microorganisms, even, and serves as comprehensive nanomaterials in more application areas. | |||
===Biosensor=== | |||
*In recent years, with the development of immobilized microorganisms technology, the microbial electrode has come up with the advantage of stable quality and relatively low cost. However, there is still no report about the use of CNT in the construction of microbial electrode. In our project, the SWNTs could be covered by protein MreB and connected with biomembrane. Thus there will be a hope to combine SWNTs with living microorganisms, and create of a new kind of coupling way of intracellular enzyme reaction and electrochemical process. | |||
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