Biomod/2011/Tianjin:Results: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 2: Line 2:
<div style="padding: 10px; width: 770px; border: 5px solid #190006;">
<div style="padding: 10px; width: 770px; border: 5px solid #190006;">
=Results=
=Results=
=='''AFM'''==
=='''CFS with microfluidics'''==
[[Image:Biomod tianjin results 1.png|center|680px|thumb|Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro.
[[Image:Biomod tianjin results 0.png|center|500px|thumb|Fig 1. Cell-free protein expression in W/O/W. Fluorescence micrograph of a W/O/W during the expression of (a) RFP as a soluble protein in the protocell cytosol, and (b) MreB-RFP, which has portioned at the hydrophobic interface. Figure (c) shows a confocal micrograph (3um thick plane) of a W/O/W, 14 h after generation. The RFP-tagged MreB polymerized, forming aggregates localized to the oil-water interface. The image was created by forming the W/O/W droplets with 100 nM BODIPY dispersed in the oil phase and MreB-RFP in the segmented phase. Fluorescence was detected in separate channels. ]]
 
 
 
=='''CFS with SWNTs'''==
==='''AFM'''===
[[Image:Biomod tianjin results 1.png|center|680px|thumb|Fig 2.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro.
a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed.
a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed.
b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated
b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated
Line 9: Line 15:
  ]]
  ]]


=='''SEM'''==
==='''SEM'''===
[[Image:Biomod tianjin results 24.png|center|600px|thumb|Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs.
[[Image:Biomod tianjin results 24.png|center|600px|thumb|Fig 3. SEM analysis of MreB-RFP cell-free aggregation with SWNTs.
a). SEM image of SWNT, the sidewall of nanotube is smooth
a). SEM image of SWNT, the sidewall of nanotube is smooth
without any absorption or aggregation.
without any absorption or aggregation.
Line 19: Line 25:
  ]]
  ]]


=='''TEM'''==
==='''TEM'''===
[[Image:Biomod tianjin results 3.png|center|600px|thumb|Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs.
[[Image:Biomod tianjin results 3.png|center|600px|thumb|Fig 4. TEM analysis of MreB-RFP cell-free aggregation with SWNTs.
a). TEM image of SWNT, the sidewall of nanotube is smooth
a). TEM image of SWNT, the sidewall of nanotube is smooth
without any absorption or aggregation.
without any absorption or aggregation.
Line 29: Line 35:
]]
]]


=='''Fluorescence micrograph'''==
==='''Fluorescence micrograph'''===
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]]
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 5. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]]




</div>
</div>

Revision as of 12:12, 31 October 2011


Home            Project            Results            Video            Team            Acknowledgements

Results

CFS with microfluidics

Fig 1. Cell-free protein expression in W/O/W. Fluorescence micrograph of a W/O/W during the expression of (a) RFP as a soluble protein in the protocell cytosol, and (b) MreB-RFP, which has portioned at the hydrophobic interface. Figure (c) shows a confocal micrograph (3um thick plane) of a W/O/W, 14 h after generation. The RFP-tagged MreB polymerized, forming aggregates localized to the oil-water interface. The image was created by forming the W/O/W droplets with 100 nM BODIPY dispersed in the oil phase and MreB-RFP in the segmented phase. Fluorescence was detected in separate channels.


CFS with SWNTs

AFM

Fig 2.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro. a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated and wound spirally onto the surface of nanotube.

SEM

Fig 3. SEM analysis of MreB-RFP cell-free aggregation with SWNTs. a). SEM image of SWNT, the sidewall of nanotube is smooth without any absorption or aggregation. b). SEM image of MreB-RFP without scaffold, protein aggregated in disorder and only discrete spherical particles were formed. c). SEM image of fusion protein at the presence of SWNT, protein aggregated and wound spirally onto the surface of nanotube.

TEM

Fig 4. TEM analysis of MreB-RFP cell-free aggregation with SWNTs. a). TEM image of SWNT, the sidewall of nanotube is smooth without any absorption or aggregation. b). TEM image of MreB-RFP without scaffold, protein aggregated in disorder and only discrete spherical particles were formed. c). TEM image of fusion protein without SWNT scaffold, protein aggregated and wound spirally onto the surface of SWNT.

Fluorescence micrograph

Fig 5. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2011/Tianjin:Results&oldid=557209"