Biomod/2011/Tianjin:Results: Difference between revisions
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=Results= | =Results= | ||
=='''AFM'''== | =='''AFM'''== | ||
[[Image:Biomod tianjin results 1.png|center| | [[Image:Biomod tianjin results 1.png|center|600px|thumb|Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro. | ||
a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. | a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. | ||
b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated | b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated | ||
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=='''SEM'''== | =='''SEM'''== | ||
[[Image:Biomod tianjin results 24.png|center| | [[Image:Biomod tianjin results 24.png|center|600px|thumb|Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs. | ||
a). SEM image of SWNT, the sidewall of nanotube is smooth | a). SEM image of SWNT, the sidewall of nanotube is smooth | ||
without any absorption or aggregation. | without any absorption or aggregation. | ||
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=='''TEM'''== | =='''TEM'''== | ||
[[Image:Biomod tianjin results 3.png|center| | [[Image:Biomod tianjin results 3.png|center|600px|thumb|Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs. | ||
a). TEM image of SWNT, the sidewall of nanotube is smooth | a). TEM image of SWNT, the sidewall of nanotube is smooth | ||
without any absorption or aggregation. | without any absorption or aggregation. | ||
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]] | ]] | ||
==''' | =='''Fluorescence micrograph'''== | ||
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]] | [[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]] | ||
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