Biomod/2011/Tianjin

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=Cell-free synthesis of actin-like cytoskeleton filaments=
=Cell-free synthesis of actin-like cytoskeleton filaments=
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MreB, an actin-like cytoskeleton in E. coli encoded by MreB gene, is essential for the maintenance of bacterial cell shape. Our project is aimed at the cell-free synthesis of MreB, and the actin homolog MreB was further characterized through both microfluidics technology and nanobiotechnology. As in vivo, MreB is rigidly linked to the cell wall. Here, MreB was cell-free expressed in vitro. Protein localization on the inner oil film could be observed in the W/O/W microdroplets produced with microfluidic technologies, which mimics the cell membrane to provide a non-polar environment. Furthermore, CNTs (carbon nanotubes) were involved as scaffolds for the auxiliary expression of actin homolog MreB. The combination of MreB with CNTs and protein aggregation were observed though the AFM (atomic force microscope) in vitro. Our research cell-free synthesizes the actin-like cytoskeleton filaments, and provides a sound microenvironment for protein synthesis, which helps to recognize, explore and create life.
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MreB, an actin-like cytoskeleton in E. coli encoded by MreB gene, is essential for the maintenance of cellular shape of non-spherical bacteria. Our project is aimed at cell-free synthesis of actin homolog MreB, which is further characterized through both microfluidics technology and nanobiotechnology. As in vivo, MreB is rigidly linked to the cell wall. Here, MreB was cell-free expressed in vitro. Protein localization on the inner oil film could be observed in W/O/W microdroplets produced with microfluidic, which mimic cell membrane to provide a non-polar environment. Furthermore, SWNTs (single-walled carbon nanotubes) were involved as scaffolds for auxiliary aggregation of actin homolog MreB. The combination of MreB with SWNT was observed under AFM (atomic force microscope). It seems that aggregated MreB winded on the carbon nanotubes. In conclusion, we synthesized the actin-like cytoskeleton filaments in vitro, and provided a sound microenvironment for MreB synthesis, which might contribute to recognizing, exploring and even creating lives.
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=Thank you=
=Thank you=

Revision as of 08:49, 29 October 2011


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Cell-free synthesis of actin-like cytoskeleton filaments

MreB, an actin-like cytoskeleton in E. coli encoded by MreB gene, is essential for the maintenance of cellular shape of non-spherical bacteria. Our project is aimed at cell-free synthesis of actin homolog MreB, which is further characterized through both microfluidics technology and nanobiotechnology. As in vivo, MreB is rigidly linked to the cell wall. Here, MreB was cell-free expressed in vitro. Protein localization on the inner oil film could be observed in W/O/W microdroplets produced with microfluidic, which mimic cell membrane to provide a non-polar environment. Furthermore, SWNTs (single-walled carbon nanotubes) were involved as scaffolds for auxiliary aggregation of actin homolog MreB. The combination of MreB with SWNT was observed under AFM (atomic force microscope). It seems that aggregated MreB winded on the carbon nanotubes. In conclusion, we synthesized the actin-like cytoskeleton filaments in vitro, and provided a sound microenvironment for MreB synthesis, which might contribute to recognizing, exploring and even creating lives.

Thank you


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