Latest revision as of 04:38, 12 March 2012

      <style type="text/css">

/* ====================

  主に全体に関わるCSS

　　　==================== */

      body {behavior: url(http://www.xs4all.nl/~peterned/htc/csshover3-source.htc);}

      .clear {clear:both;}

body.mediawiki {

                       font-size: 14px;

background-color:#707070; background-position: center center; background-attachment: fixed; background-repeat: no-repeat; font-family: Calibri, Verdana, helvetica, sans-serif; }

               h1 {
                       padding: 0px 20px 5px 20px;
                       font-size: 34px;
                       font-weight: bold;
               }
               h2 {
                       padding: 20px 20px 5px 20px;
                       font-size: 25px;
                       color: #0083eb;
                       text-decoration: none;
                       font-weight: bold;}
                       border: none;
               h2 a {
                       color: #eb8300;
               }
               h3 {
                       padding: 20px 20px 5px 20px;
                       font-size: 20px;
                       color: #000;
                       font-decoration: none;
                       font-weight: bold;
               }
               h1.firstHeading {

display: none;

p { text-align: justify; } a:link { color: #00a5ea; text-decoration: none } a:visited { color:#00a5ea; text-decoration: none } a:hover {

                       color: #eb8300;

text-decoration: none } a:active { color:#f29400; text-decoration: none } #bodyContent {

                       width: 970px;
                       margin: 0px 0px 0px;
                       background-color:#ffffff;
                       border-width: 0px 1px 0px 1px;
                       border-color: #000000;
               }
               #content {
                       padding-left: 0px; width: 970px;
               }

table#team_members {

                       text-align: justify;
                       border: 0;
               }

table#team_members h2, table#team_members h3 {

                       clear: both;
               }

#content * a:hover {

                       text-decoration:none;
               }

#main_wrapper {

                       position:absolute;
                       left:0px;
                       top:20px;

margin-top: 0; width: 969px; height: 221px; align: center; border-style: solid;

                       border-color: white;

} /* ====================

  メニューの画像を変更できる部分
  ==================== */

#header {

                       position:relative;
                       left:0px;
                       top:0px;

margin-top: 0; width: 969px; height: 221px; align: left; background-color: #FFFFFF;

                       background-image: url(http://openwetware.org/images/e/e0/NEW_header.jpg);

} /* ====================

  以下、特殊なclassに適用される
  ==================== */

#navigation { position:absolute;

                       left:18px; top:155px; width:1200px; height:69px;

z-index:100;

                       background-color: transparent;
                       float: left;

color: #0000FF; } #super_main_wrapper {

                       position:absolute;
                       left:0px;
                       top:227px;

width: 975px; align: center; background-color: #ede8e2;

                       heigth: auto;
               }
               #SubWrapper {
                       width: 645px;
                       padding: 0px;
                       border-left:4px solid #ede8e2;
                       float: left;
                       margin-top: 0px;
                       background-color: #ede8e2;
               }
               #SubWrapper * p, #SubWrapper p  {
                       padding: 0 20px;
                       text-align: justify;
                       font-size: 12px;
               }
               #SubWrapper * h3, #SubWrapper h3 {
                       padding-top: 10px;
                       font-size: 18px;
               }
               #news {
                       width: 322px;
                       margin-top: 0px;
                       float: left;
                       background-color: #d8d5d0;
                       border-right:4px solid #ede8e2;
               }
               #news p {
                       padding: 0 20px 20px 20px;
                       text-align: justify;
                       font-size: 12px;
               }
               #news h3 {
                       padding: 10px 20px;
                       font-size: 18px;
               }
               #mission_box {
                       width:650px;
                       float: left;
               }
               #team_box, #heartbeat_box, #notebook_box, #parts_box, #gallery_box, #sponsors_box, .boxy {
                       width:215px;
                       float: left;
                       padding: 10px 0 0 0;
               }
               div.tleft {
                       border-width: 0px;
                       margin:0;
                       padding:0;
                       border-color:transparent;
               }

/* ====================

  ここからプルダウン周辺のデザイン
  ==================== */

menu * {

margin: 0; padding: 0; }

menu {

behavior: url(http://www.xs4all.nl/~peterned/htc/csshover3-source.htc);  font-family: calibri, verdana, sans-serif;

       font-color: #ffffff;

font-size: 19px; background-color: transparent; float:left; padding: 12px 0 0 0; }

menu ul {

float: left; list-style: none; }

menu ul li {

background-color:transparent;

       position:relative;

float:left; list-style: none; padding: 10px 20px 0 0;

       font-weight: bold;
       width: auto;

}

menu a {

color: #FFFFFF; display: inline; text-decoration: none; }

menu a:visited {

color:#FFFFFF; text-decoration: none }

menu a:hover {

color: #00a5ea; }

menu ul li ul {

display: none; position: absolute; left: 0px;

       width: 155px;
       heigth: 1%;

font-size: 19px; opacity: 0.8; list-style: none;

       top: 30px;
       padding-top: 20px;
       z-index:500;

}

menu ul li:hover ul {

display: inline;

       background-position: bottom;

}

menu ul li ul li {

width: 100%; list-style: none;

       background-color: #000;
       margin: -1px;
       padding: 0px 0 0 5px;
       display: inline;

}

</style>

<!--[if IE]> <style type="text/css">

SubWrapper {width: 645px;}
news {width: 317px;}
super_main_wrapper {position:static;}
navigation {left: 15px;}
menu ul li ul {left: -2px; top: 22px;}

</style> <![endif]--> <div id="main_wrapper">

 <div id="header">

<div id="navigation"> <div id="menu" style="position:static"> <ul> <li><a class="aMain" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo">Home</a></li> <li><a class="aTeam" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Team/Students">Team</a></li> <li><a class="aProject" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project">Project</a>  <li><font color="#ffffff">Results</font> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Results">Experiments</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Simulations">Simulations</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Achievements/DNA_Devices">DNA Design</a></li> </ul></li>  <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Achievements">Achievements</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Future_works">Future works</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Notebook/Protocols">Protocols</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Notebook/Lab.notebook">Notes</a></li> <li><a class="aNotebook" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Sponsors/">Sponsors</a></li> <li><a class="aSitemap" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Sitemap">Sitemap</a></li>

</ul> </div> </div>  </div> </div>  <script type="text/javascript">

/***********************************************

CSS Vertical List Menu- by JavaScript Kit (www.javascriptkit.com)
Menu interface credits: http://www.dynamicdrive.com/style/csslibrary/item/glossy-vertical-menu/
This notice must stay intact for usage
Visit JavaScript Kit at http://www.javascriptkit.com/ for this script and 100s more

- - - - /

var menuids=new Array("verticalmenu") //Enter id(s) of UL menus, separated by commas var submenuoffset=-2 //Offset of submenus from main menu. Default is -2 pixels.

function createcssmenu(){ for (var i=0; i<menuids.length; i++){

 var ultags=document.getElementById(menuids[i]).getElementsByTagName("ul")
   for (var t=0; t<ultags.length; t++){
   var spanref=document.createElement("span")

spanref.className="arrowdiv" spanref.innerHTML="  " ultags[t].parentNode.getElementsByTagName("a")[0].appendChild(spanref)

   ultags[t].parentNode.onmouseover=function(){
   this.getElementsByTagName("ul")[0].style.left=this.parentNode.offsetWidth+submenuoffset+"px"
   this.getElementsByTagName("ul")[0].style.display="block"
   }
   ultags[t].parentNode.onmouseout=function(){
   this.getElementsByTagName("ul")[0].style.display="none"
   }
   }
 }

} if (window.addEventListener) window.addEventListener("load", createcssmenu, false) else if (window.attachEvent) window.attachEvent("onload", createcssmenu) </script>  </html>

TeamJapan Tokyo developed an autonomous micrometer-sized molecular robot, “DNA ciliate”, toward the construction of a highly functional molecular robot like a living cell.

Project overview

A living cell is an ultimate highly functional molecular robot. The high functionality results from not only its various functional molecules but also its “micrometer-sized” body that has enough space to possess the molecules. However, the sizes of already-developed molecular nanomachines are too small to include many functional molecules. Toward the construction of highly functional molecular machines and molecular robots, it is required to construct molecular robots with large (i.e., micrometer-sized) bodies. Here, we propose an autonomous micrometer-sized molecular robot “DNA ciliate”.

<html><body> <iframe width="450" height="263" src="http://www.youtube.com/embed/aze2N57tcng" frameborder="0" allowfullscreen></iframe> <br><center>This is our YouTube video.</center> </body></html>

<html> <a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project"><img src="http://openwetware.org/images/2/2c/Biomod2011_Team_Tokyo_111101Biomod_three_mode_02_R.jpg" border=1 width="470" height="353"></a><br> <font size="3" color="#000066"><i><strong> <font size="3" color="#009933">“DNA ciliate”</font>is an autonomous micrometer-sized molecular robot that has three independent functional modes. </strong></i></font><br><br> </html>

DNA ciliate

“DNA ciliate” is an autonomous micrometer-sized molecular robot, which is inspired by water microorganisms, ciliates. A natural ciliate has a micrometer-sized body with cilia and achieves various functions such as autonomous motion with the cilia, phototaxis, etc. Similarly, the DNA ciliate has a micrometer-sized body with many DNAs as cilia, and it can switch three different modes in response to its external environment: the free moving mode, the track walking mode, and the light-irradiated gathering mode. (See below)

Three independent functional modes of DNA ciliate

The DNA ciliate can move around in a broad range of space using the free moving mode. This mode utilizes the Brownian motion of the DNA ciliate.
See more

In the track walking mode, the DNA ciliate walk directionally along a single-stranded DNA track on a plate using the cleaving activity of deoxyribozymes attached on the body of the DNA ciliate. The deoxyribozymes cleave substrate DNA strands arrayed as the track, and help the DNA ciliate move to the region of uncleaved substrate DNA step-by-step.
See more

The DNA ciliates gather at a specific area where an ultraviolet (UV) light is irradiated; this is the light-irradiated gathering mode. To achieve this mode, a UV-switching DNA system based on the cis-trans isomerization of azobenzenes was designed.
See more

@@ Line 1: / Line 1: @@
 {{HEAD_1}}
 __NOTOC__
-<!--この上の呪文たちは必ず各ページのコピペすること-->
+:<br><i>TeamJapan Tokyo developed an autonomous micrometer-sized molecular robot, “DNA ciliate”, toward the construction of a highly functional molecular robot like a living cell.</i><br><br>
 =Project overview=
+<table width="100%">
+ <tr valign="top">
+  <td width="450px" style="padding-left: 20px">
+&nbsp;&nbsp;A living cell is an ultimate highly functional molecular robot. The high functionality results from not only its various functional molecules but also its “micrometer-sized” body that has enough space to possess the molecules. However, the sizes of already-developed molecular nanomachines are too small to include many functional molecules. Toward the construction of highly functional molecular machines and molecular robots, it is required to construct molecular robots with large (i.e., micrometer-sized) bodies. Here, we propose an autonomous micrometer-sized molecular robot “DNA ciliate”.
+  </td>
+  <td width="450px" style="padding-left: 20px">
 <HTML><body>
-<table width="100%">
+<iframe width="450" height="263" src="http://www.youtube.com/embed/aze2N57tcng" frameborder="0" allowfullscreen></iframe>
+<br><center>This is our YouTube video.</center>
+</body></HTML>
+  </td>
+ </tr>
   <tr>
- <td>
+  <td align="center"><!--valign="top"-->
-<i>We developed an autonomous micrometer-sized molecular robot,“DNA ciliate,toward a highly functional molecular robot like living cells.</i><br><br>
+<HTML>
-&nbsp;A living cell is an ultimate highly functional molecular robot. The high functionality is caused by not only its various nanometer-sized functional molecules but also its “micrometer-sized” body that has enough space to possess the molecules. However, the size of already-developed molecular nano-robots is too small to include multiple functions. Toward highly functional molecular robots, it is required to construct molecular robots with micrometer-sized body. Here, we propose an autonomous DNA-based molecular robot “DNA ciliate”. A natural ciliate has a micrometer-sized body with cilia and achieves various functions such as autonomous motion with the cilia, phototaxis, etc. DNA ciliate has a micrometer-sized body with many DNAs as cilia, and it can switch three different modes in response to its external environment: the free moving mode, tracks walking mode, and light-irradiated gathering mode. We believe our concept will promote the construction of highly functional molecular robots like cells in future.</td>
+<a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project"><img src="http://openwetware.org/images/2/2c/Biomod2011_Team_Tokyo_111101Biomod_three_mode_02_R.jpg" border=1 width="470" height="353"></a><br>
-　　<td align="top"><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Over_view"><img src="http://openwetware.org/images/9/9c/DNA_ciliate概念図.png" border=1 width="400" height="300"></a>
+<font size="3" color="#000066"><i><strong>
-   <font size="3" color="#000066"><i><strong>
+<font size="3" color="#009933">“DNA ciliate”</font>is an autonomous micrometer-sized molecular robot that has three independent functional modes.
-<font size="3" color="#00ff66">“DNA ciliate”</font> has three independent functional modes.
+</strong></i></font><br><br>
-</strong></i></font></td>
+</HTML>
-  </tr>
+  </td>
+  <td style="padding-left: 30px">
+==DNA ciliate==
+<HTML><Center>
+<a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project#The_body_of_the_DNA_ciliate"><img
+src="http://openwetware.org/images/8/8c/DNA_ciliate_body.jpg" border=1 width="250px"></a>
+</Center></HTML>
+“DNA ciliate” is an autonomous micrometer-sized molecular robot, which is inspired by water microorganisms, ciliates. A natural ciliate has a micrometer-sized body with cilia and achieves various functions such as autonomous motion with the cilia, phototaxis, etc. Similarly,<B> the DNA ciliate has a micrometer-sized body with many DNAs as cilia</B>, and it can<B> switch three different modes</B> in response to its external environment:<B> the free moving mode, the track walking mode, and the light-irradiated gathering mode.</B> (See below)
+  </td>
+  </tr>
 </table>
-<center>
-<iframe width="420" height="315" src="http://www.youtube.com/embed/uejCuQWG07A" frameborder="0" allowfullscreen></iframe>
-</center>
-</body></HTML>
-==DNA ciliate body==
-{|
-|-
-|width="320px"|[[Image:DNA ciliate body.jpg|light|250px|DNA ciliates'body]]
-|
-:To create DNA ciliate, a micrometer-sized body and its motor are indispensable. We chose DNA as a material for the motor because DNA is the most suitable material that is nanometer-sized and easy to be attached to micrometer-sized objects by various surface modifications. The micrometer-sized body is required to be micrometer-sized, homogeneous, and easy to attach DNA.
-|}
+==Three independent functional modes of DNA ciliate==
 <table width="100%">
-<tr align="center">
+<tr align="center" style="padding-left: 10px">
-<td>
+<HTML>
-==1. Free moving mode==
+ <td style="padding: 0px 10px">
-[[Image:Free moving mode figure.jpg|300px]]</td>
+<a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project#1._Free_moving_mode"><img src="http://openwetware.org/images/d/d4/Biomod2011_Team_Tokyo_111101Biomod_FreeMovingMode_3_title.gif" border=1 width="300"></a>
-<td>
+ </td>
-==2. Track walking mode==
+ <td style="padding: 0px 10px">
-[[Image:Track walking mode figure.jpg|300px]]</td>
+<a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project#2._Track_walking_mode"><img src="http://openwetware.org/images/a/a5/Biomod2011_Team_Tokyo_111101Biomod_TrackWalkingMode_3_title.gif" border=1 width="300"></a>
-<td>
+ </td>
-==3. Light-irradiated gathering mode==
+ <td style="padding: 0px 10px">
-[[image:Tokyo-gathering1.png|300px]]<td>
+<a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project#3._Light-irradiated_gathering_mode"><img src="http://openwetware.org/images/9/9e/Biomod2011_Team_Tokyo_111101Biomod_Right-irradiatedGatheringMode_3_title.gif" border=1 width="300"></a>
+ </td>
+</HTML>
 </tr>
-<tr>
+<tr valign="top" align="center">
-<td>[[Biomod/2011/TeamJapan/Tokyo/Project/Free_moving_mode|The free moving mode]] is utilizing Brownian motion. We changed the size of DNA ciliate and observed the DNA ciliate movement by Brownian motion.</td>
+<td style="padding: 0px 10px">
-<td>[[Biomod/2011/TeamJapan/Tokyo/Project/Track_walking_mode|The track walking mode]] is utilizing deoxyribozyme activity for substrate. We checked deoxyribozyme activity and simulated DNA ciliate's movement on micrometer-sized track and confirmed the track walking mode by simulation.</td>
+The DNA ciliate can move around in a broad range of space using the free moving mode. This mode utilizes the Brownian motion of the DNA ciliate.
-<td>[[Biomod/2011/TeamJapan/Tokyo/Project/Light_irradiated_gathering_mode|The light-irradiated mode]] is utilizing UV-switching DNA. We checked the "UV-switching system" and confirmed DNA ciliate trapping at one spot.</td>
+<br>[[Biomod/2011/TeamJapan/Tokyo/Project#1. Free moving mode|See more]]
-</tr>
+</td>
+<td style="padding: 0px 10px">
+In the track walking mode, the DNA ciliate walk directionally along a single-stranded DNA track on a plate using the cleaving activity of deoxyribozymes attached on the body of the DNA ciliate. The deoxyribozymes cleave substrate DNA strands arrayed as the track, and help the DNA ciliate move to the region of uncleaved substrate DNA step-by-step.
+<br>[[Biomod/2011/TeamJapan/Tokyo/Project#2. Track walking mode|See more]]
+</td>
+<td style="padding: 0px 10px">
+The DNA ciliates gather at a specific area where an ultraviolet (UV) light is irradiated; this is the light-irradiated gathering mode. To achieve this mode, a UV-switching DNA system based on the cis-trans isomerization of azobenzenes was designed.
+<br>[[Biomod/2011/TeamJapan/Tokyo/Project#3. Light-irradiated gathering mode|See more]]
+</td>
 </tr>
 </table>
-==1. Free moving mode==
-{|
-|-
-|width="300px" valign="top"|
-[[Image:Free moving mode figure.jpg|250px|center]]
-:[[Biomod/2011/TeamJapan/Tokyo/Project/Free_moving_mode|The free moving mode]] is utilizing Brownian motion. We changed the size of DNA ciliate and observed the DNA ciliate movement by Brownian motion.
-|}
-==2. Track walking mode==
-{|
-|-
-|width="300px"|[[Image:Track walking mode figure.jpg|left|300px]]
-|
-:[[Biomod/2011/TeamJapan/Tokyo/Project/Track_walking_mode|The track walking mode]] is utilizing deoxyribozyme activity for substrate. We checked deoxyribozyme activity and simulated DNA ciliate's movement on micrometer-sized track and confirmed the track walking mode by simulation.
-|}
-==3. Light-irradiated gathering mode==
-{|
-|-
-|width="320px"|[[image:Tokyo-gathering1.png|300px|center]]
-|
-:[[Biomod/2011/TeamJapan/Tokyo/Project/Light_irradiated_gathering_mode|The light-irradiated mode]] is utilizing UV-switching DNA. We checked the "UV-switching system" and confirmed DNA ciliate trapping at one spot.
-|}

Biomod/2011/TeamJapan/Tokyo: Difference between revisions

Latest revision as of 04:38, 12 March 2012

Project overview

DNA ciliate

Three independent functional modes of DNA ciliate

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

research

Tools