Biomod/2011/TUM/TNT:Notebook/Biomod/2011/TUM/TNT/2011/08/24: Difference between revisions

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*for purification, batches of identical probes were combined
*for purification, batches of identical probes were combined
*labeled oligos were resuspended in 100µl 0.5xTBE 11mM MgCl2 (50µl for the_U 218 and the_U 255)
*labeled oligos were resuspended in 100µl 0.5xTBE 11mM MgCl2 (50µl for the_U 218 and the_U 255)
* [[concentrations]]
* further see [[Determined Concentrations of Labeled Oligos]]
<br>
<br>
*prepared solutions for determining absorption coefficients of unlabeled oligos at nanodrop
*prepared solutions for determining absorption coefficients of unlabeled oligos at nanodrop

Revision as of 07:18, 30 August 2011

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Banden für The U - Rampe: 1565

Bild gespeichert unter: Biomod-Ordner, Rechner: Gelbilder

  • Reihen von links nach rechts: 1kb-ladder, scaffold 7650 20nMol, Bm1, Bm2
    • jeweils Bande 1: Bande, die langsamer gelaufen ist!
    • jeweils Bande 2: Bande, die schneller gelaufen ist!

labeling oligos with fluorophores

  • labeling using terminal transferase according to protocol in BioNanoWiki, but 2x the usual volumes (final volume: 80µl)
  • all reactions were accomplished twice, but for the_U 218 and the_U 225 only one reaction was successful
  • labeled oligos:


  • the_U 175 with Atto550_ddCTP helix 4
  • the_U 218 with Atto647N_ddUTP helix 23


  • the_U 197 with Atto550_ddCTP helix 8
  • the_U 225 with Atto647N_ddUTP helix 29


  • the_U 183 with Atto550_ddCTP helix 5
  • the_U 204 with Atto647N_ddUTP helix 20



  • prepared solutions for determining absorption coefficients of unlabeled oligos at nanodrop
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