Biomod/2011/TUM/TNT:Notebook/Biomod/2011/TUM/TNT/2011/08/24: Difference between revisions

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** the_U 175 with Atto550_ddCTP      helix 4
** the_U 175 with Atto550_ddCTP      helix 4
** the_U 218 with Atto647N_ddUTP      helix 23
** the_U 218 with Atto647N_ddUTP      helix 23
[break]
[br]
** the_U 197 with Atto550_ddCTP      helix 8
** the_U 197 with Atto550_ddCTP      helix 8
** the_U 225 with Atto647N_ddUTP      helix 29
** the_U 225 with Atto647N_ddUTP      helix 29
[break]
[br]
** the_U 183 with Atto550_ddCTP      helix 5
** the_U 183 with Atto550_ddCTP      helix 5
** the_U 204 with Atto647N_ddUTP      helix 20
** the_U 204 with Atto647N_ddUTP      helix 20

Revision as of 11:12, 24 August 2011

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Banden für The U - Rampe: 1565

Bild gespeichert unter: Biomod-Ordner, Rechner: Gelbilder

  • Reihen von links nach rechts: 1kbleadder, scaffold 7650 20nMol, Bm1, Bm2
    • jeweils Bande 1: Bande, die langsamer gelaufen ist!
    • jeweils Bande 2: Bande, die schneller gelaufen ist!

labeling oligos with fluorophores

  • labeling using terminal transferase according to protocol in BioNanoWiki, but doubled volume (final volume: 80µl)
  • labeled oligos:
    • the_U 175 with Atto550_ddCTP helix 4
    • the_U 218 with Atto647N_ddUTP helix 23

[br]

    • the_U 197 with Atto550_ddCTP helix 8
    • the_U 225 with Atto647N_ddUTP helix 29

[br]

    • the_U 183 with Atto550_ddCTP helix 5
    • the_U 204 with Atto647N_ddUTP helix 20



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