# Biomod/2011/TUM/TNT/Results

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The analysis program is a [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Evaluation_of_Data matlab script] which searches for spots in the red and the green movie and plots the intensities over time to identify bleaching events. Only those plots where the acceptor bleaches first and the donor bleaches afterwards are useful to calculate the FRET-efficiency (see figure 9).
The graph shows the intensities of the donor and the acceptor and in addition the intensity of the FRET-events. As one can see the intensity of the donor rises as soon as the acceptor bleaches. After some while the donor bleaches too. From that the FRET-efficiency can be calculated.
The analysis program is a [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Evaluation_of_Data matlab script] which searches for spots in the red and the green movie and plots the intensities over time to identify bleaching events. Only those plots where the acceptor bleaches first and the donor bleaches afterwards are useful to calculate the FRET-efficiency (see figure 9).
The graph shows the intensities of the donor and the acceptor and in addition the intensity of the FRET-events. As one can see the intensity of the donor rises as soon as the acceptor bleaches. After some while the donor bleaches too. From that the FRET-efficiency can be calculated.
- First we measured the FRET-efficiencies for the [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page#BM12_to_BM14:_Fluorophore-labeled_Structures_for_FRET_Measurements.2C_including_Adapters_for_Immobilisation BM14 structure] without any intercalator or groove binder as a control and afterward we measured the same structure with 4.8µM spermine (corresponding to one molecule every 7bp). The FRET-efficiencies were plotted in figure 9.
[[Image:Schöner_FRET_Verlauf.PNG|400px|thumb|Fig. 9 Intensities of donor and acceptor]]
[[Image:Schöner_FRET_Verlauf.PNG|400px|thumb|Fig. 9 Intensities of donor and acceptor]]
+ First we measured the FRET-efficiencies for the [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page#BM12_to_BM14:_Fluorophore-labeled_Structures_for_FRET_Measurements.2C_including_Adapters_for_Immobilisation BM14 structure] without any intercalator or groove binder as a control and afterward we measured the same structure with 4.8µM spermine (corresponding to one molecule every 7bp). The FRET-efficiencies were plotted in figure 10.

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