# Biomod/2011/TUM/TNT/Results

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Survey of results

Survey of results

We successfully designed a structure that folds properly with high yields [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Folding_.26_Purification (link)] and is suitable for observing the structural deformations. Comprehensive TEM analysis yielded insights into global structural deformations and allowed for statistical evaluation of angle and length distributions dependent on DNA binder concentrations [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#TEM_Image_Analysis (link)]. Our structure could be labeled with fluorescent dyes and a huge variety of different approaches to fluorescence measurements was tested. In single molecule measurements FRET events could be observed. [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Fluorescence_Measurements (link)]. Based on these experimental data and also our structure simulations and calculations, we gained new insights into the structural properties of DNA origamis especially with regards to binding of small molecules [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Discussion (link)]. We successfully designed a structure that folds properly with high yields [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Folding_.26_Purification (link)] and is suitable for observing the structural deformations. Comprehensive TEM analysis yielded insights into global structural deformations and allowed for statistical evaluation of angle and length distributions dependent on DNA binder concentrations [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#TEM_Image_Analysis (link)]. Our structure could be labeled with fluorescent dyes and a huge variety of different approaches to fluorescence measurements was tested. In single molecule measurements FRET events could be observed. [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Fluorescence_Measurements (link)]. Based on these experimental data and also our structure simulations and calculations, we gained new insights into the structural properties of DNA origamis especially with regards to binding of small molecules [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results#Discussion (link)]. -

Folding & Purification

Folding & Purification

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NumberMeanVarianceSEMNumber of particlesMean angle [degree]VarianceStandard error of the means
ctrl7159.3124.8300.180642978negative control7159.34.80.18
ctrl pretwisted42421.1198.1610.396347765positive control (pretwisted)42421.18.20.40
Spermine 0.42 µM10198.8644.7930.150157669Spermine 0.42 µM10198.94.80.15
Spermine 1.34 µM18099.663 5.7590.1354099Spermine 1.34 µM18099.7 5.80.14
EtBr 0.69 µM1569.169 5.0360.403186678Ethidium bromide 0.69 µM1569.2 5.00.40
EtBr 0.74 µM47211.059 5.5770.256697629Ethidium bromide 0.74 µM47211.1 5.60.26
EtBr 2.27 µM22310.877 5.5310.370363066Ethidium bromide 2.27 µM22310.9 5.50.37
EtBr 2.4 µM4317.474 7.8390.377567259Ethidium bromide 2.4 µM4317.5 7.80.38
DAPI 432 nM3756.0277 5.37360.277491511DAPI 432 nM3756.0 5.40.28
DAPI 144 nM3257.5568 4.13490.229362984DAPI 144 nM3257.6 4.10.23
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