Biomod/2011/TUM/TNT/Methods/TEM
<html>
<style>
- column-one { display:none; width:0px;}
.container{background-color: #f5f5f5; margin-top:50px} .OWWNBcpCurrentDateFilled {display: none;}
- content { width: 0px; margin: 0 auto auto 0; padding: 1em 1em 1em 1em; align: center;}
- column-content {width: 0px; float: left; margin: 0 0 0 0;padding: 0;}
.firstHeading {display:none; width:0px;}
- globalWrapper{ width:1280px;}
div.tleft { border-bottom-width: 0em; border-left-width: 0px; border-right-width: em; border-top-width: 0em; clear: left; float: left; margin-right: 0.5em;
}
div.tnone { border-bottom-width: 0em; border-left-width: 0px; border-right-width: em; border-top-width: 0em; }
- column-one {display:none; width:0px;background-color: #ffffff;}
- content{ margin: 0 0 0 0; padding: 1em 1em 1em 1em; position: center; width: 800px;background-color: #f5f5f5; }
.container{ width: 800px; margin: auto; background-color: #ffffff; text-align:justify; font-family: helvetica, arial, sans-serif; color:#555555; margin-top:25px; }
- bodyContent{ width: 1267px; align: center; background-color: #ffffff;}
- column-content{width: 1280px;background-color: #f5f5f5;}
.firstHeading { display:none;width:0px;background-color: #ffffff;}
- header{position: center; width: 800px;background-color: #ffffff;}
- footer{position: center;}
div.thumb { border-bottom-color: rgb(255, 255, 255); border-bottom-style: solid; border-left-color: rgb(255, 255, 255); border-left-style: solid; border-right-color: rgb(255, 255, 255); border-right-style: solid; border-top-color: rgb(255, 255, 255); border-top-style: solid; margin-bottom: 0.5em; width: auto;
}
div.tright { border-bottom-width: 0em; border-left-width: 0px; border-right-width: 0px; border-top-width: 0em; clear: right; float: right;
}
body {
font-family: helvetica, arial, sans-serif; color: black;
background:#f5f5f5;
}
ul#topnav {
margin: 0 0 0 0px ; padding: 0 0 0 0px; float: left; width: 800px; list-style: none; position: relative;
font-family: helvetica, arial, sans-serif;
font-size: 12px; background: #f5f5f5;
height: 5px;
} ul#topnav li { float: left; margin: 0; padding: 0; border-right: 1px solid #ffffff; /*--Divider for each parent level links--*/ } ul#topnav li a { padding: 11px 15px; display: block; color: #333333; text-decoration: none; } ul#topnav li:hover { background: #f5f5f5;} /*--Notice the hover color is on the list item itself, not on the link. This is so it can stay highlighted even when hovering over the subnav--*/
ul#topnav li span { float: left; padding: 15px 0; position: absolute; left: 0; top:30px; display: inline; /*--Hide by default--*/ width: 800px; background: #cccccc; color: #333333; /*--Bottom right rounded corner--*/ -moz-border-radius-bottomright: 5px; -khtml-border-radius-bottomright: 5px; -webkit-border-bottom-right-radius: 5px; /*--Bottom left rounded corner--*/ -moz-border-radius-bottomleft: 5px; -khtml-border-radius-bottomleft: 5px; -webkit-border-bottom-left-radius: 5px; } ul#topnav li:hover span { display: block; } /*--Show subnav on hover--*/ ul#topnav li span a { display: inline;} /*--Since we declared a link style on the parent list link, we will correct it back to its original state--*/ ul#topnav li span a:hover {text-decoration: underline;}
- abstractandlinks{
display:block; width:800px; background-color:#f5f5f5; text-align:justify;font-family: helvetica, arial, sans-serif; color:#555555; margin-top:0px; }
- innerBox
{ display: block; padding: 5px; }
- abstract{
font-family: helvetica, arial, sans-serif; width: 490px; margin-top: 20px; padding:0px 5px; float: left;background-color:#f5f5f5; ; }
- links{
font-family: helvetica, arial, sans-serif; width: 290px; margin-top: 20px; padding:0px 5px; float: left; background-color:#f5f5f5;; } .clear { clear:both; }
- tour{
font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; margin-top: 1em, margin-bottom: 1em; float: left;background:#f5f5f5; font-size: 2em; }
- struktur{
font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; float: left;background:#f5f5f5; }
- toctitle {align: left; text-align: left; }
- table.toc {background-color:"#5f5f5f";align: left; text-align: left;}
- toc #toctitle, .toc #toctitle, #toc .toctitle, .toc .toctitle {
text-align: left;
}
- toc, .toc, .mw-warning {
background-color: #f5f5f5; border-bottom-color: rgb(170, 170, 170); border-bottom-style: solid; border-bottom-width: 0px; border-left-color: rgb(170, 170, 170); border-left-style: solid; border-left-width: 0px; border-right-color: rgb(170, 170, 170); border-right-style: solid; border-right-width: 0px; border-top-color: rgb(170, 170, 170); border-top-style: solid; border-top-width: 0px; font-size: 105%; padding-bottom: 5px; padding-left: 5px; padding-right: 5px; padding-top: 5px; align: left; text-align: left; display: left
}
</style>
<head>
<script type="text/javascript"> var fullDates = new Array('08/11/2011','08/12/2011','08/16/2011','08/17/2011','08/23/2011','08/24/2011','08/25/2011','08/29/2011','08/30/2011','08/31/2011','09/01/2011','09/02/2011','09/06/2011','09/12/2011','09/13/2011','09/14/2011','09/15/2011','09/16/2011','09/19/2011','09/20/2011','09/21/2011','09/27/2011','09/28/2011','09/29/2011','09/30/2011','10/05/2011','10/06/2011','10/07/2011','10/10/2011','10/12/2011','10/13/2011','10/14/2011','10/17/2011','10/18/2011','10/19/2011','10/20/2011','10/21/2011','10/26/2011','10/27/2011','10/28/2011','10/30/2011');
$(document).ready(function() {
$("ul#topnav li").hover(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav
} ,
$("ul#topnav li").oneclick(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'; 'display' : 'inline'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav
} ,
function() { //on hover out...
$(this).css({ 'background' : 'none'}); //Ditch the background
//$(this).find("span").hide(); //Hide the subnav });
}); </script>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.3/jquery.min.js"></script>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script> <script type="text/javascript"> $(document).ready(function(){ $('#lside1').mouseover(function() { $('#lside1').stop().animate({backgroundPosition: '0px 0px'}, 1000); }); $('#lside1').mouseleave(function() { $('#lside1').stop().animate({backgroundPosition: '-250px 0px'}, 1000); }); $('#lside2').mouseover(function() { $('#lside2').stop().animate({backgroundPosition: '0px 0px'}, 1000); }); $('#lside2').mouseleave(function() { $('#lside2').stop().animate({backgroundPosition: '-250px 0px'}, 1000); }); $('#lside3').mouseover(function() { $('#lside3').stop().animate({backgroundPosition: '0px 0px'}, 1000); }); $('#lside3').mouseleave(function() { $('#lside3').stop().animate({backgroundPosition: '-250px 0px'}, 1000); }); }); </script>
<style type="text/css">
#side1 { width: 250px; height: 100px; position: relative; top: 0px; left: 20px;}
#lside1 { width: 250px; height: 100px; background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat; position: absolute; top: 0px; left: 0px;}
#side2 { width: 250px; height: 100px; position: relative; top: 0px; left: 20px;}
#lside2 { width: 250px; height: 100px; background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat; position: absolute; top: 0px; left: 0px;}
#side3 { width: 250px; height: 100px; position: relative; top: 0px; left: 20px;}
#lside3 { width: 250px; height: 100px; background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat; position: absolute; top: 0px; left: 0px;}
</style>
<title>
TUM NanU - Home
</title> </head>
<body background-color="#F0F0F0"> <!-- Header--> <div class="container">
<div id="header"> <img src="http://openwetware.org/images/5/5c/Header2.png" /> </div>
<!--Menue--> <ul id="topnav">
<li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Home">Home</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Project">Project</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results">Results & Discussion</a> </li> <li style="background-color: #cccccc"><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods"><b>Methods</b></a> <span> <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Folding">Folding</a>| <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Purification">Purification</a>| <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Labeling">Labeling</a>| <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#TEM">TEM</a>| <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods#Fluorescence_Microscopy">Fluorescence Microscopy</a> </span> </li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team">Team</a></li>
<li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA">Labbook</a><!--Subnav Starts Here-->
<li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Extras">Extras</a> </li>
</ul> <p> <br> </p> <p> <br> </p> <p> <br> </p>
</body> </html>
TEM
http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/Methods/TEM&action=edit
Transmission electron microscopy (TEM) is an imaging technique to observe thin specimen. An electron beam interacts with the specimen while passing and is detected by a sensor, in this case a CCD camera.
DNA consists of atoms with small atomic numbers. This leads to only weak contrast. For fixation on the samples to enhance the contrast, uranyl formiate is applied as negative stain.
As DNA is light material, the samples were stained with uranyl acetate to enhance contrast. Electrons can pass through the origami-structure and are absorbed or scattered by the stain. Read more about preparing samples for transmission electron microscope.
The images taken for this project were made at the BioNano TEM facility (CM 100, Philips, Amsterdam, NL) at a high voltage of 100.0 kV and a nominal magnification of 28500. Images were 4x averaged and integrated to reduce noise.
Besides confirming the proper folding of our structure, the main purpose of the TEM measurements was to provide data on twists and lengths of the structures, dependent on concentrations of the DNA binders. These angle and length measurements were accomplished with the the free tool imagej.
http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/Methods/TEM&action=edit