Biomod/2011/TUM/TNT/Methods/Folding: Difference between revisions

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Folding of an origami is a critical step. Besides the question, whether a designed structure folds at all, the yield and the fraction of misfolded structures are also important. The yield determines, which concentrations could be applied for further experiments. If significant amounts of misfoldings do occur, thourough purification is needed, which in turn goes hand in hand with reduced yield due to purification losses. For these reasons, it was not only aimed to design a structure, that can be expected to fold easily, but also some efforts were expended to test different thermal folding ramps and check the efficiency of folding. <br>
<h2>Folding procedure</h2>
<h3>Prestocks</h3>
Several hundreds of staples have to be mixed for a folding batch. Those staples, which are frequently used together for different variants of the structure, were mixed in prestocks. Later on, for small modifications of the structure, only the appropriate prestock needs to be remixed, while all other parts could be used again. Prestocks may vary in the number of included staples, but in one prestock, all staples are equally concentrated (usually 10µl 100µM staple are mixed).
<h3>Working stocks</h3>




Revision as of 06:33, 25 October 2011

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