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<title>

       TUM NanU - Home

</title> </head>

   <div id="header"> <img src="http://openwetware.org/images/5/5c/Header2.png" /> </div>

<ul id="topnav">

   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Home">Home</a></li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Project">Project</a>        
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   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results">Results & Discussion</a>        
   </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods">Methods</a></li>

   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team">Team</a></li>
<li style="background-color: #cccccc"><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA"><b>Labbook</b></a><!--Subnav Starts Here-->
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           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA">Main Page</a>||
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page">Structure</a>|
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures">TEM</a>|
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/TIRF">Fluorescence Microscopy</a>|

           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Glossary">Glossary</a>
        </span>
   </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Extras">Extras</a>
       
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http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit

summary of all prepared and observed grids

All dilutions were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.

BIOMOD1 grid box

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<tr bgcolor="#f5f5f5"><td><b>grid number   </b></td><td><b>prepared   </b></td><td><b>annotations   </b></td><td><b>observed   </b></td><td><b>annotation</b></td></tr>

</table> </html>

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<tr bgcolor="#f5f5f5"><td valign="top"><b>grid number   </b></td><td valign="top"><b>prepared   </b></td><td valign="top"><b>annotations   </b></td><td valign="top"><b>observed   </b></td><td valign="top"><b>annotation</b></td></tr>

<tr bgcolor="#f5f5f5"><td valign="top">A1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A2</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A3</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A4</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A5</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to high structure concentration, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A6</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25</td><td valign="top">oct. 28st</td><td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A7</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25</td><td valign="top">oct. 27th</td><td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A8</td><td valign="top">oct. 20th</td><td valign="top">BM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25 </td><td valign="top">oct. 28th<td valign="top">no origamis on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A9</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A10</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B1</td><td valign="top">oct. 5th</td><td valign="top">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">grid was fine</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">too high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">stain was good</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top">BM24 15_65 1:25 without any DNA binders</td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B10</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain and dilution was good</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 dilution 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was bad</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML dilution 1:20 without any DNA binders </td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top">BM24 2DH3ML 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C9</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C10</td><td valign="top">oct. 27th</td><td valign="top">BM2 1:25 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">fine grid, 60 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D1</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D2</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top">BM2 1:25without any DNA binders </td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65), 1:20 diluted with FOB20</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65) 1:5 without any DNA binders</td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than 2D6 and 2D8, 40 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">strong background</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E4</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)<td valign="top">oct. 26th</td><td valign="top">fine grid</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E5</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">weak stain</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E6</td><td valign="top">oct. 28st</td><td valign="top">BM24 (15_65), 1:5</td><td valign="top">oct. 28st</td><td valign="top">fine grid, 100 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">oct. 31th</td><td valign="top">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</td><td valign="top">oct. 31th</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">oct. 31th</td><td valign="top">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</td><td valign="top">oct. 31th</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">2</td><td valign="top">3</td><td valign="top">4</td><td valign="top">5</td></tr>

</table> </html>

Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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