Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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<tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66  images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66  images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top">BM2 without any DNA binders, 1:25</td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/3/36/20111020_theU_BM24_2B8.png">BM24 15_65 1:25 without any DNA binders</a></td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/3/36/20111020_theU_BM24_2B8.png">BM24 15_65 1:25 without any DNA binders</a></td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid, 3 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid, 3 images</td></tr>

Revision as of 23:36, 2 November 2011

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TEM

http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit


All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.

Contents

BIOMOD1 Grid Box

grid number   prepared   annotations   observed   annotation
B2sep. 16thBM2 1:5 control without DNA-binderssep. 21thfine grid
B5sep. 16thBM2 1:5 with 100 µM sperminesep. 21thstructures accumulated
C2sep. 16thBM2 1:5 with 25 µM ethidium bromidesep. 21thmassive deformation of the structures
C3sep. 16thBM2 1:5 with 75 µM ethidium bromidesep. 21thbroken grid
C4sep. 16thBM2 1:5 with 250 µM ethidium bromidesep. 21thdestroyed structures, not enough structures

BIOMOD2 Grid Box

grid number   prepared   annotations   observed   annotation
A1oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 62 images
A2oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 63 images
A3oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 60 images
A4oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to low dilution, 64 images
A5oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to low dilution, 63 images
A6oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 28stno origamis on the grid, no images
A7oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 27thno origamis on the grid, no images
A8oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 28thno origamis on the grid, no images
A9oct. 20thBM2 1:25 without any DNA bindersoct. 21thgood stain and dilution, 40 images
A10oct. 20thBM2 1:25 without any DNA bindersoct. 21thgood stain and dilution, 60 images
B1oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 12thgrid was fine, 60 images
B2oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thtoo high dilution, 25 images
B3oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 13thgood stain and structure concentration, 36 images
B4oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 14thgood stain but slightly to low structure concentration, 35 images
B5oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)45
B6oct. 20thBM2 without any DNA binders, 1:25oct. 21thgood stain and dilution, 66 images
B7oct. 20thBM2 without any DNA binders, 1:25oct. 21thgood stain and dilution, 80 images
B8oct. 20thBM24 15_65 1:25 without any DNA bindersoct. 20thbad stain and to high dilution
B9oct. 20thBM24 2DH3ML 1:25 without any DNA binders oct. 20thbad stain, no structures on the grid, 3 images
B10oct. 21thBM24 15_65 1:20 without any DNA bindersoct. 21thgood stain but slightly to high dilution, 61 images
C1oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 14thgood stain but dilution slightly to low, 32 images
C2oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 14thgood stain but dilution slightly to low, 31 images
C3oct. 10thBM2 1:40 without any DNA binders45
C4oct. 10thBM2 1:40 without any DNA binders45
C5oct. 10thBM2 1:40 without any DNA binders45
C6oct. 21thBM24 15_65 dilution 1:20 without any DNA bindersoct. 21thstain was bad and to high dilution, 17 images
C7oct. 21thBM24 2DH3ML dilution 1:20 without any DNA binders oct. 21thstain was good but dilution slightly to high, 70 images
C8oct. 21thBM24 2DH3ML 1:20 without any DNA bindersoct. 21thstain was good but dilution slightly to high
C9oct. 27thBM2 1:25 without any DNA bindersoct. 27good stain but to high dilution, 60 images
C10oct. 27thBM2 1:25 without any DNA bindersoct. 27thfine grid, 60 images
D1oct. 12thBM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thgood stain and dilution, 74 images
D2oct. 12thBM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 12thgood stain and dilution, 89 images, chosen for class average
D3oct. 12thBM2 1:25without any DNA binders oct. 12thgood stain and dilution, 50 images
D4oct. 14thBM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)oct. 20thgood stain and structur concentration but cluster, 62 images
D5oct. 14thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 20thstain and dilution was good, cluster, 61 images
D6oct. 27thBM24 (15_65), 1:20 diluted with FOB20oct. 27thgood stain but slightly to high dilution, 22 images
D7oct. 27thlost the grid before sample preparation45
D8oct. 27thBM24 (15_65) 1:10 without any DNA bindersoct. 27thlow sample concentration, 24 images
D9oct. 27thBM24 (15_65) 1:10 without any DNA binders4,stain was good but dilution slightly to low
D10oct. 28stBM24 (15_65) 1:5 without any DNA bindersoct. 28st better sample concentration than D6 and D8, 60 images
E1oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thgood dilution, bad staining, 49 images
E2oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thgood dilution but strong background, 50 images
E3oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid, 60 images
E4oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid, 50 images
E5oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)/a>oct. 26thgood dilution but weak stain, 60 images
E6oct. 28stBM24 (15_65), 1:5oct. 28stfine grid, 413 images
E7oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31thgrid was fine, 112 images
E8oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31thexcellent grid, 80 images
E9nov. 1stBM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)nov. 1stgood grid, 111 images
E10nov. 1stBM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)nov. 1stfine grid, 131 images
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