Biomod/2011/TUM/TNT/LabbookA/Setting up folding reactionsng reactions: Difference between revisions
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==working stock== | ==working stock== | ||
Working stocks are mixed from the appropriate prestocks. Different amounts of staples in each prestock are taken into account by adjusting the volumes of the prestocks. So if 109 staples are mixed in the core prestock, then 109µl of this prestock are used to mix the working stock. If the total volume of all prestocks mixed in this way is less than 200µl, then ddH<sub>2</sub> is added up to a final volume of 200µl working stock. <br> | Working stocks are mixed from the appropriate prestocks. Different amounts of staples in each prestock are taken into account by adjusting the volumes of the prestocks. So if 109 staples are mixed in the core prestock, then 109µl of this prestock are used to mix the working stock. If the total volume of all prestocks mixed in this way is less than 200µl, then ddH<sub>2</sub>O is added up to a final volume of 200µl working stock. <br> | ||
For example, the working stock for the structure BM2 consists of: | For example, the working stock for the structure BM2 consists of: | ||
* 109µl prestock core | * 109µl prestock core |
Revision as of 06:44, 25 October 2011
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For example, the working stock for the structure BM2 consists of: |