Biomod/2011/TUM/TNT/LabbookA/2011/TIRF: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(6 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{TUMNanUHeaderLabbookA}}
{{TUMNanUHeaderLabbookA}}
<div id="struktur">
<div id="struktur">
<h1>summary of all fluorescence microscopy related experiments</h1>


<h1>All Experiments related to Dyes and Fluorescence Microscopy</h1>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/11 Aug 11th] Measured absorption and emission spectra of Atto 550 and Atto 647N together with DNA binders<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/17 Aug 17th] Measuring the absorption coefficients and concentrations of single stranded labled DNA<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/23 Aug 23th] Preparing DAPI concentration series for the helix MH255/256<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/24 Aug 24th] Labeling oligos with fluorophores<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/25 Aug 25th] Determined Concentrations of Labeled Oligos<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/08/30 Aug 30th]  Measurement of DAPI concentration series for the helix MH255/256 in Real Time PCR<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/01 Sep 1st] Real time PCR with MH_255_647N and MH_256_550 adding ethidium bromide and spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/06 Sep 6th] Photometric spectroscopy of MH_255_647 and MH_256_550 with EtBr<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/13 Oct 18th] labeled oligonucleotides for twisted structure BM25<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/13 Sep. 13th] Fluorescence microscopic ensemble measurement of the labeled structure BM5_5/20<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/13 Sep. 13th] Fluorescence microscopic ensemble measurement of the labeled structure BM5_5/20<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/14 Sep 14th] preparation of samples for TIRF bulk measurements with spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/14 Sep 14th] preparation of samples for TIRF bulk measurements with spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/15 Sep 15th] Fluorescence microscope bulk measurements with spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/15 Sep 15th] Fluorescence microscope bulk measurements with spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/19 Sep 19th] Labeled oligonucleotides & Determination of absorption coefficient of unlabeled the_U_172 and the_U_173<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/20 Sep 20th] Measured concentrations of labeled oligonucleotides<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/29 Sep 29th] determine suitable concentrations for for single molecule fluorescence microscopy<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/29 Sep 29th] determine suitable concentrations for for single molecule fluorescence microscopy<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/30 Sep 30th] Single molecule fluorescence microscopy measurement of BM14_5/20 and BM21_172/173<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/09/30 Sep 30th] Single molecule fluorescence microscopy measurement of BM14_5/20 and BM21_172/173<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/06 Oct 6th] Single molecule fluorescence microscopy with sample BM21_172/173<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/06 Oct 6th] Single molecule fluorescence microscopy with sample BM21_172/173<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/13 Oct 13th] Single molecule fluorescence microscopy measurement of BM21_172/173 with/without spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/13 Oct 13th] Single molecule fluorescence microscopy measurement of BM21_172/173 with/without spermine<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/18 Oct 18th] Fluorescence microscope with BM12_4/29 to calculate the distance<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/18 Oct 18th] Fluorescence microscope with BM12_4/29 to calculate the distance [http://openwetware.org/wiki/Image:Green_BM12_1to5000_Spermin_1to21.zip Spermine 0.42 µM green] &  labeled oligonucleotides for twisted structure BM25 [http://openwetware.org/wiki/Image:Green_BM12_1to5000_Spermin_1to7.zip Spermine 1.34 µM green]<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/19 Oct 19th] Camera calibration<br>
[http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/10/19 Oct 19th] Camera calibration<br>

Latest revision as of 23:20, 2 November 2011

<html>

  <style>
  1. column-one { display:none; width:0px;}

.container{background-color: #f5f5f5; margin-top:50px} .OWWNBcpCurrentDateFilled {display: none;}

  1. content { width: 0px; margin: 0 auto auto 0; padding: 1em 1em 1em 1em; align: center;}
  1. column-content {width: 0px; float: left; margin: 0 0 0 0;padding: 0;}

.firstHeading {display:none; width:0px;}

  1. globalWrapper{ width:1280px;}

div.tright { border-bottom-width: 0em; border-left-width: 0px; border-right-width: 0px; border-top-width: 0em; clear: right; float: right;

}

div.tleft { border-bottom-width: 0em; border-left-width: 0px; border-right-width: em; border-top-width: 0em; clear: left; float: left; margin-right: 0.5em;

}

div.tnone { border-bottom-width: 0em; border-left-width: 0px; border-right-width: em; border-top-width: 0em; }

  1. column-one {display:none; width:0px;background-color: #ffffff;}
  1. content{ margin: 0 0 0 0; padding: 1em 1em 1em 1em; position: center; width: 800px;background-color: #f5f5f5; }

.container{ width: 800px; margin: auto; background-color: #ffffff; text-align:justify; font-family: helvetica, arial, sans-serif; color:#555555; margin-top:25px; }

  1. bodyContent{ width: 1267px; align: center; background-color: #ffffff;}
  1. column-content{width: 1280px;background-color: #f5f5f5;}

.firstHeading { display:none;width:0px;background-color: #ffffff;}

  1. header{position: center; width: 800px;background-color: #ffffff;}
  1. footer{position: center;}

<style type="text/css"> body {

   font-family: helvetica, arial, sans-serif;
   color: black;

background:#f5f5f5;

 }
ul#topnav {

margin: 0 0 0 0px ; padding: 0 0 0 0px; float: left; width: 800px; list-style: none; position: relative;

       font-family: helvetica, arial, sans-serif;

font-size: 12px; background: #f5f5f5;

       height: 5px;

} ul#topnav li { float: left; margin: 0; padding: 0; border-right: 1px solid #ffffff; /*--Divider for each parent level links--*/ } ul#topnav li a { padding: 11px 15px; display: block; color: #333333; text-decoration: none; } ul#topnav li:hover { background: #f5f5f5;} /*--Notice the hover color is on the list item itself, not on the link. This is so it can stay highlighted even when hovering over the subnav--*/

ul#topnav li span { float: left; padding: 15px 0; position: absolute; left: 0; top:30px; display: inline; /*--Hide by default--*/ width: 800px; background: #cccccc; color: #333333; /*--Bottom right rounded corner--*/ -moz-border-radius-bottomright: 5px; -khtml-border-radius-bottomright: 5px; -webkit-border-bottom-right-radius: 5px; /*--Bottom left rounded corner--*/ -moz-border-radius-bottomleft: 5px; -khtml-border-radius-bottomleft: 5px; -webkit-border-bottom-left-radius: 5px; } ul#topnav li:hover span { display: block; } /*--Show subnav on hover--*/ ul#topnav li span a { display: inline;} /*--Since we declared a link style on the parent list link, we will correct it back to its original state--*/ ul#topnav li span a:hover {text-decoration: underline;}


  1. abstractandlinks{

display:block; width:800px; background-color:#f5f5f5; text-align:justify;font-family: helvetica, arial, sans-serif; color:#555555; margin-top:0px; }

  1. innerBox

{ display: block; padding: 5px; }

  1. abstract{

font-family: helvetica, arial, sans-serif; width: 490px; margin-top: 20px; padding:0px 5px; float: left;background-color:#f5f5f5; ; }

  1. links{

font-family: helvetica, arial, sans-serif; width: 290px; margin-top: 20px; padding:0px 5px; float: left; background-color:#f5f5f5;; } .clear { clear:both; }

  1. tour{

font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; margin-top: 1em, margin-bottom: 1em; float: left;background:#f5f5f5; font-size: 2em; }

  1. struktur{

font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; float: left;background:#f5f5f5; }

  1. toctitle {align: left; text-align: left; }
  2. table.toc {background-color:"#5f5f5f";align: left; text-align: left;}
  3. toc #toctitle, .toc #toctitle, #toc .toctitle, .toc .toctitle {

text-align: left;

}
  1. toc, .toc, .mw-warning {

background-color: #f5f5f5; border-bottom-color: rgb(170, 170, 170); border-bottom-style: solid; border-bottom-width: 0px; border-left-color: rgb(170, 170, 170); border-left-style: solid; border-left-width: 0px; border-right-color: rgb(170, 170, 170); border-right-style: solid; border-right-width: 0px; border-top-color: rgb(170, 170, 170); border-top-style: solid; border-top-width: 0px; font-size: 105%; padding-bottom: 5px; padding-left: 5px; padding-right: 5px; padding-top: 5px; align: left; text-align: left; display: left

}


</style>

<head>

<script type="text/javascript">


$(document).ready(function() {

$("ul#topnav li").hover(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav

} ,

$("ul#topnav li").oneclick(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'; 'display' : 'inline'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav

} ,

function() { //on hover out...


$(this).css({ 'background' : 'none'}); //Ditch the background

//$(this).find("span").hide(); //Hide the subnav });

}); </script>


<title>

       TUM NanU - Home   

</title> </head>

<body background-color="#F0F0F0"> <!-- Header--> <div class="container">

   <div id="header"> <img src="http://openwetware.org/images/5/5c/Header2.png" /> </div> 
   

<!--Menue--> <ul id="topnav">

   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Home">Home</a></li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Project">Project</a>        
      </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results">Results & Discussion</a>        
   </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods">Methods</a></li>
      
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team">Team</a></li>
<li style="background-color: #cccccc"><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA"><b>Labbook</b></a><!--Subnav Starts Here-->
       <span>
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA">Main Page</a>||
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/Structure_page">Structure</a>|
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures">TEM</a>|
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA/2011/TIRF">Fluorescence Microscopy</a>|
           <a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Glossary">Glossary</a>
        </span>
   </li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Extras">Extras</a>
       
  </li>

</ul> <p> <br> </p> <p> <br> </p> <p> <br> </p> </html>

<html>

<html> <head> <script type= "text/javascript">/*<![CDATA[*/ var skin = "monobook"; var stylepath = "/skins"; var wgArticlePath = "/wiki/$1"; var wgScriptPath = ""; var wgScript = "/index.php"; var wgVariantArticlePath = false; var wgActionPaths = []; var wgServer = "http://openwetware.org"; var wgCanonicalNamespace = ""; var wgCanonicalSpecialPageName = false; var wgNamespaceNumber = 0; var wgPageName = "Biomod/2011/TUM/TNT/LabbookA/2011"; var wgTitle = "Biomod/2011/TUM/TNT/LabbookA/2011 var wgAction = "view"; var wgArticleId = "103708"; var wgIsArticle = true; var wgUserName = "Alexander Hipp"; var wgUserGroups = ["*", "user", "autoconfirmed"]; var wgUserLanguage = "en"; var wgContentLanguage = "en"; var wgBreakFrames = false; var wgCurRevisionId = "543703"; var wgVersion = "1.13.2"; var wgEnableAPI = true; var wgEnableWriteAPI = false; var wgMWSuggestTemplate = "http://openwetware.org/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}"; var wgDBname = "owwdb"; var wgSearchNamespaces = [0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 100, 101, 110, 111]; var wgMWSuggestMessages = ["with suggestions", "no suggestions"]; var wgRestrictionEdit = []; var wgRestrictionMove = []; var wgAjaxWatch = {"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching..."}; /*]]>*/</script>

<script type="text/javascript" src="/skins/common/wikibits.js?164"><!-- wikibits js --></script> <!-- Head Scripts --> <script type="text/javascript" src="/js/calendar.js"></script> <script type="text/javascript"> function lnCalendar(){

 var calm1 = new CalendarPopup('m1');
 var wikiPage = 'Biomod/2011/TUM/TNT/LabbookA';
 var redirPage = '/wiki/Special:Redir/'+wikiPage;
 var fullDates = new Array('08/11/2011','08/17/2011','08/23/2011','08/24/2011','08/25/2011','08/29/2011','08/30/2011','09/01/2011','09/02/2011','09/06/2011','09/12/2011','09/13/2011','09/14/2011','09/15/2011','09/16/2011','09/19/2011','09/20/2011','09/21/2011','09/27/2011','09/28/2011','09/29/2011','09/30/2011','10/05/2011','10/06/2011','10/07/2011','10/10/2011','10/12/2011','10/13/2011','10/14/2011','10/17/2011','10/18/2011','10/19/2011','10/20/2011','10/21/2011','10/26/2011','10/27/2011','10/28/2011','10/30/2011','10/31/2011');
 calm1.setDateFormat('yyyy/MM/dd');
 calm1.setCssPrefix('OWWNB');
 calm1.setSingleMonth('8');
 calm1.setYear('2011');
 calm1.setReadOnly('N');
 calm1.setTodayText('');
 calm1.setUrlPrefix(redirPage);
 for(i = 0; i < fullDates.length; i++)calm1.addFilledDates(fullDates[i]);
 calm1.showCalendar('m1');
 var calm2 = new CalendarPopup('m2');

('08/11/2011','08/12/2011','08/16/2011','08/17/2011','08/23/2011','08/24/2011','08/25/2011','08/29/2011','08/30/2011','09/01/2011','09/02/2011','09/06/2011','09/12/2011','09/13/2011','09/14/2011','09/15/2011','09/16/2011','09/19/2011','09/20/2011','09/21/2011','09/27/2011','09/28/2011','09/29/2011','09/30/2011','10/05/2011','10/06/2011','10/07/2011','10/10/2011','10/12/2011','10/13/2011','10/14/2011','10/17/2011','10/18/2011','10/19/2011','10/20/2011','10/21/2011','10/26/2011','10/27/2011','10/28/2011','10/30/2011','10/31/2011');

 calm2.setDateFormat('yyyy/MM/dd');
 calm2.setCssPrefix('OWWNB');
 calm2.setSingleMonth('9');
 calm2.setYear('2011');
 calm2.setReadOnly('N');
 calm2.setTodayText('');
 calm2.setUrlPrefix(redirPage);
 for(i = 0; i < fullDates.length; i++)calm2.addFilledDates(fullDates[i]);
 calm2.showCalendar('m2');
 var calm3 = new CalendarPopup('m3');

('08/11/2011','08/12/2011','08/16/2011','08/17/2011','08/23/2011','08/24/2011','08/25/2011','08/29/2011','08/30/2011','09/01/2011','09/02/2011','09/06/2011','09/12/2011','09/13/2011','09/14/2011','09/15/2011','09/16/2011','09/19/2011','09/20/2011','09/21/2011','09/27/2011','09/28/2011','09/29/2011','09/30/2011','10/05/2011','10/06/2011','10/07/2011','10/10/2011','10/12/2011','10/13/2011','10/14/2011','10/17/2011','10/18/2011','10/19/2011','10/20/2011','10/21/2011','10/26/2011','10/27/2011','10/28/2011','10/30/2011','10/31/2011');

 calm3.setDateFormat('yyyy/MM/dd');
 calm3.setCssPrefix('OWWNB');
 calm3.setSingleMonth('10');
 calm3.setYear('2011');
 calm3.setReadOnly('N');
 calm3.setTodayText('');
 calm3.setUrlPrefix(redirPage);
 for(i = 0; i < fullDates.length; i++)calm3.addFilledDates(fullDates[i]);
 calm3.showCalendar('m3');

} </script> <script type="text/javascript">addOnloadHook( lnCalendar )</script> <script type="text/javascript">function CalendarPageConfirmCreate(date, url){var answer = confirm("Create entry for "+date+"?");if (answer){window.location = url;}}</script> <script type="text/javascript" src="/skins/common/ajax.js?164"></script> <script type="text/javascript" src="/ext/AjaxShowEditors/AjaxShowEditors.js"></script> <script type="text/javascript" src="/skins/common/ajaxwatch.js?164"></script> <script type="text/javascript" src="/skins/common/mwsuggest.js?164"></script> <script type="text/javascript" src="/index.php?title=-&amp;action=raw&amp;smaxage=0&amp;gen=js&amp;useskin=monobook"><!-- site js --></script>


<style type="text/css">/*<![CDATA[*/ @import "/index.php?title=MediaWiki:Common.css&amp;usemsgcache=yes&amp;action=raw&amp;ctype=text/css&amp;smaxage=18000"; @import "/index.php?title=MediaWiki:Monobook.css&amp;usemsgcache=yes&amp;action=raw&amp;ctype=text/css&amp;smaxage=18000"; @import "/index.php?title=-&amp;action=raw&amp;gen=css&amp;maxage=18000&amp;smaxage=0&amp;ts=20111012100110&amp;useskin=monobook"; @import "/index.php?title=User:Alexander_Hipp/monobook.css&amp;action=raw&amp;ctype=text/css"; /*]]>*/</style> <script type="text/javascript" src="/index.php?title=User:Alexander_Hipp/monobook.js&amp;action=raw&amp;ctype=text/javascript"></script> </head>

</html>

All Experiments related to Dyes and Fluorescence Microscopy

Aug 11th Measured absorption and emission spectra of Atto 550 and Atto 647N together with DNA binders
Aug 17th Measuring the absorption coefficients and concentrations of single stranded labled DNA
Aug 23th Preparing DAPI concentration series for the helix MH255/256
Aug 24th Labeling oligos with fluorophores
Aug 25th Determined Concentrations of Labeled Oligos
Aug 30th Measurement of DAPI concentration series for the helix MH255/256 in Real Time PCR
Sep 1st Real time PCR with MH_255_647N and MH_256_550 adding ethidium bromide and spermine
Sep 6th Photometric spectroscopy of MH_255_647 and MH_256_550 with EtBr
Oct 18th labeled oligonucleotides for twisted structure BM25
Sep. 13th Fluorescence microscopic ensemble measurement of the labeled structure BM5_5/20
Sep 14th preparation of samples for TIRF bulk measurements with spermine
Sep 15th Fluorescence microscope bulk measurements with spermine
Sep 19th Labeled oligonucleotides & Determination of absorption coefficient of unlabeled the_U_172 and the_U_173
Sep 20th Measured concentrations of labeled oligonucleotides
Sep 29th determine suitable concentrations for for single molecule fluorescence microscopy
Sep 30th Single molecule fluorescence microscopy measurement of BM14_5/20 and BM21_172/173
Oct 6th Single molecule fluorescence microscopy with sample BM21_172/173
Oct 13th Single molecule fluorescence microscopy measurement of BM21_172/173 with/without spermine
Oct 18th Fluorescence microscope with BM12_4/29 to calculate the distance Spermine 0.42 µM green & labeled oligonucleotides for twisted structure BM25 Spermine 1.34 µM green
Oct 19th Camera calibration