Biomod/2011/TUM/TNT/LabbookA/2011/10/18: Difference between revisions
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===labeled oligonucleotides for twisted structure the_U v2=== | ===labeled oligonucleotides for twisted structure the_U v2=== | ||
according to [http://openwetware.org/wiki/Labeling_oligonucleotides_with_fluorophores standard procedures] <br> | according to [http://openwetware.org/wiki/Labeling_oligonucleotides_with_fluorophores standard procedures] <br> | ||
labeled staple 110 (helix | labeled staple 110 (helix 4) with ddUTP Atto 550 <br> | ||
labeled staple 164 (helix | labeled staple 164 (helix 29) with ddUTP Atto 647N <br> | ||
finally, labeled oligonucleotides were dissolved in 50µl TE buffer <br> | finally, labeled oligonucleotides were dissolved in 50µl TE buffer <br> | ||
<br> | <br> | ||
Revision as of 09:09, 18 October 2011
labeled oligonucleotides for twisted structure the_U v2
according to standard procedures
labeled staple 110 (helix 4) with ddUTP Atto 550
labeled staple 164 (helix 29) with ddUTP Atto 647N
finally, labeled oligonucleotides were dissolved in 50µl TE buffer
for concentration measurements, absorption coefficients of unlabeled staples 110 and 164 were determined
ε260(staple 110) = 3.5·105 M-1cm-1
ε260(staple 164) = 3.3·105 M-1cm-1
