Biomod/2011/TUM/TNT/LabbookA/2011/10/18: Difference between revisions
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Manuel Hora (talk | contribs) (New page: ===labeled oligonucleotides for twisted structure the_U v2=== according to [http://openwetware.org/wiki/Labeling_oligonucleotides_with_fluorophores standard procedures] <br> labeled staple...) |
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for concentration measurements, absorption coefficients of unlabeled staples 110 and 164 were determined <br> | for concentration measurements, absorption coefficients of unlabeled staples 110 and 164 were determined <br> | ||
ε<sub>260</sub>(staple 110) = | ε<sub>260</sub>(staple 110) = 3.5·10<sup>5</sup> M<sup>-1</sup>cm<sup>xx</sup> <br> | ||
ε<sub>260</sub>(staple 164) = | ε<sub>260</sub>(staple 164) = xxx·10<sup>xx</sup> M<sup>-1</sup>cm<sup>xx</sup> <br> |
Revision as of 08:07, 18 October 2011
labeled oligonucleotides for twisted structure the_U v2
according to standard procedures
labeled staple 110 (helix xxx) with ddUTP Atto xxx
labeled staple 164 (helix xxx) with ddUTP Atto xxx
finally, labeled oligonucleotides were dissolved in 50µl TE buffer
for concentration measurements, absorption coefficients of unlabeled staples 110 and 164 were determined
ε260(staple 110) = 3.5·105 M-1cmxx
ε260(staple 164) = xxx·10xx M-1cmxx