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Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.1s
Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.1s


[[Image:Histogramm_0.5s_excitation.tif]] <br>
Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.5s




Revision as of 02:20, 25 October 2011

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single molecule fluorescence microscopy with sample BM21_172/173

  • The last single molecule fluorescence microscopy measurement on 29.09.11 resulted in only few detectable FRET events with seemingly randomly distributed FRET efficiencies. Therefore the sample preparation is optimized (Immobilizing structures with biotin oligos for TIRF) and the framerate settings during measurements are modified. The framerate is set to:
    • 50ms/frame, 500 frames/video (same setting as used before)
    • 100ms/frame, 300 frames/video
    • 500ms/frame, 200 frames/video
    • 1s/frame, 100 frames/video

results


Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.05s



Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.1s



Histogram showing number of detected FRET events in intervals of FRET efficiencies; excitation time 0.5s


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