Biomod/2011/TUM/TNT/LabbookA/2011/09/16: Difference between revisions

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==TIRF measurements from 15th September==
could not be processed, since data were not saved properly for yet unknown reasons, no complete data sets available <br>
measurement will be repeated
|}
|}


__NOTOC__
__NOTOC__
[[category:OWWLabNotebookV1]]
[[category:OWWLabNotebookV1]]

Revision as of 07:09, 19 September 2011

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<head> <title>

       TUM NanU - Labbook

</title> </head> <body> <body background-color="#F0F0F0"> <!-- Header--> <div class="container">

   <div id="header"> <img src="http://openwetware.org/images/5/5c/Header2.png" /> </div> 
   

<!--Menue--> <ul id="topnav">

   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Home">Home</a></li>
   <li>
       <a href="#">Project</a>
       <!--Subnav Starts Here-->
       <span>
           <a href="#">The Idea</a> |
           <a href="#">The Plan</a> |
           <a href="#">The Video</a>
       </span>
       <!--Subnav Ends Here-->
   </li>
   <li><a href="#">Results</a>

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           <a href="#">Structure</a> 
           <a href="#">FRET</a> 
           <a href="#">....</a>
       </span>
       <!--Subnav Ends Here-->

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   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT">Testpage</a></li>
   <li><a href="http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team">Team</a></li>
   <li><a href="http://openwetware.org/wiki/Tnt_-_Labbook">Labbook</a><!--Subnav Starts Here-->
       <span>
           <a href="#">Week I</a> 
           <a href="#">Week II</a> 
           <a href="#">Week III</a>
       </span>
       <!--Subnav Ends Here-->
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   <li><a href="#">Outlook</a></li>
   <li><a href="#">Extras</a><span>
           <a href="#">How to do a stop-motion-video</a> 
           <a href="#">How to make ...</a> 
           <a href="http://openwetware.org/index.php?title=Tnt_-_Labbook&action=submit">Edit</a>
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Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

prepared grids for TEM

according to standard protocol

each sample contained BM2 purified by filtration and diluted 1/5 with FOBxM 20mM MgCl2 samples:

  • BM2 control without DNA-binders
  • BM2 with 10µM spermine
  • BM2 with 30µM spermine
  • BM2 with 100µM spermine
  • BM2 with 25µM ethidium bromide
  • BM2 with 75µM ethidium bromide
  • BM2 with 250µM ethidium bromide


TIRF measurements from 15th September

could not be processed, since data were not saved properly for yet unknown reasons, no complete data sets available
measurement will be repeated