Biomod/2011/TUM/TNT/LabbookA/2011/09/06: Difference between revisions
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* looks like there are residual staples in solution and the leading band is slower as the scaffold. Marty said this is normal, when purified samples are put on a gel. | * looks like there are residual staples in solution and the leading band is slower as the scaffold. Marty said this is normal, when purified samples are put on a gel. | ||
* new purification with less initial concentration and folding buffer as washing buffer | * new purification with less initial concentration and folding buffer as washing buffer | ||
* look at samples in the TEM as soon as possible! | * look at samples in the TEM as soon as possible! (should be repaired tommorow) | ||
==Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2== | ==Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2== |
Revision as of 23:37, 6 September 2011
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Size exclusion filter purificationFolded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) filtered in Amicon (100 kDa) with 0.5 TBE + 11mM MgCl2Sample filtering
Ran a 50 ml 2% Agarose-Gel with EtBr
Gel picture
Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2Sample filtering
Ran a 125 ml 2% Agarose-Gel with EtBr
Gel pictureinsert gel picture ConclusionPhotometric spectroscopy of MH255_647 and MH256_550 adding EtBrTo compare results with RT-PCR sample preparationpreliminary considerations
stocks
samplesLabled DNA
Control: Unlabled DNA
Hybidisation:
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