Biomod/2011/TUM/TNT/LabbookA/2011/09/06: Difference between revisions
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* spinned for 5 minutes at 14000 rcf | * spinned for 5 minutes at 14000 rcf | ||
* turned the filter and sinned the purified sample into a new Epi for 2 minutes at 1000 rcf | * turned the filter and sinned the purified sample into a new Epi for 2 minutes at 1000 rcf | ||
* | * all five flow through bufer filled into separate Epi (the buffer, which came through the filter; if purification with spin columns works, this should only contain staples) | ||
===Ran a 125 ml 2% Agarose-Gel with EtBr=== | ===Ran a 125 ml 2% Agarose-Gel with EtBr=== |
Revision as of 08:03, 6 September 2011
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Size exclusion filter purificationFolded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) filtered in Amicon (100 kDa) with 0.5 TBE + 11mM MgCl2Sample filtering
Ran a 50 ml 2% Agarose-Gel with EtBr
Gel picture
Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2Sample filtering
Ran a 125 ml 2% Agarose-Gel with EtBr
Gel pictureinsert gel picture Photometric spectroscopy of MH255_647 and MH256_550 adding EtBrTo compare results with RT-PCR sample preparationpreliminary considerations
stocks
samplesLabled DNA
Control: Unlabled DNA
Hybidisation:
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