Biomod/2011/TUM/TNT/LabbookA/2011/09/06: Difference between revisions
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=Photometric spectroscopy of MH255_647 and MH256_550 adding ErBr= | =Photometric spectroscopy of MH255_647 and MH256_550 adding ErBr= | ||
To compare results with RTPCR | To compare results with RTPCR | ||
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=== samples === | === samples === | ||
====Labled DNA==== | ====Labled DNA==== | ||
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====Control: Unlabled DNA==== | ====Control: Unlabled DNA==== | ||
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Revision as of 08:01, 6 September 2011
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Size exclusion filter purificationFolded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) filtered in Amicon (100 kDa) with 0.5 TBE + 11mM MgCl2Sample filtering
Ran a 50 ml 2% Agarose-Gel with EtBr
Gel picture
Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2Sample filtering
Ran a 125 ml 2% Agarose-Gel with EtBr
Gel pictureinsert gel picture Photometric spectroscopy of MH255_647 and MH256_550 adding ErBrTo compare results with RTPCR sample preparationpreliminary considerations
stocks
samplesLabled DNA
Control: Unlabled DNA
Hybidisation:
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