Biomod/2011/TUM/TNT/LabbookA/2011/09/06: Difference between revisions
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=== samples === | === samples === | ||
====Labled DNA==== | |||
{| class="wikitable" border="1" | |||
|- | |||
! concentration EtBr (in Units of K<sub>D</sub>) | |||
! EtBr Stock [µL] | |||
! MH255_647N 280nM [µL] | |||
! MH256_550 710nM [µL] | |||
! buffer (0,5 TBE, 11mM MgCl<sub>2</sub>) | |||
|- | |||
| 0 | |||
| 0 | |||
| 3,6 | |||
| 1,4 | |||
| 95 | |||
|- | |||
| 0.044 | |||
| 1,07 (50µM stock) | |||
| 3,6 | |||
| 1,4 | |||
| 93,93 | |||
|- | |||
| 0.4 | |||
| 9,6 (50µM stock) | |||
| 3,6 | |||
| 1,4 | |||
| 85,4 | |||
|- | |||
| 7.6 | |||
| 45.6 (200µM stock) | |||
| 3,6 | |||
| 1,4 | |||
| 49,4 | |||
|- | |||
|} | |||
Revision as of 06:03, 6 September 2011
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||
Size exclusion filter purificationFolded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) filtered in Amicon (100 kDa) with 0.5 TBE + 11mM MgCl2Sample filtering
Ran a 50 ml 2% Agarose-Gel with EtBr
Gel pictureinsert jpeg!!!
Folded sample BM_1_1565 (23.8.2011) in Amicon (100 kDa) with 1 x FOBXM + 20 mM MgCl2Sample filtering
Ran a 125 ml 2% Agarose-Gel with EtBrPhotometric spectroscopy of MH255_647 and MH256_550 adding ErBrTo compare results with RTPCR sample preparationpreliminary considerations
stocks
samplesLabled DNA
|