Biomod/2011/TUM/TNT/LabbookA/2011/09/06: Difference between revisions
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Manuel Hora (talk | contribs) (Autocreate 2011/09/06 Entry for Tnt_-_Labbook) |
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== | ==Size exclusion filter purification== | ||
===Folded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) were filtered in the following manner=== | |||
- 50 µl from both samples were mixed and put into a Amicon filter | |||
- 400 µl buffer added | |||
- spinned for 5 minutes at 14000 rcf | |||
- 400 µl buffer added | |||
- spinned for 5 minutes at 14000 rcf | |||
- 400 µl buffer added | |||
- spinned for 5 minutes at 14000 rcf | |||
- turned the filter and sinned the purified sample into a new Epi for 2 minutes at 1000 rcf | |||
- 1 x and 2 x filtered buffer filled into separate Epi (the buffer, which came through the filter; if purification with spin columns works, this should only contain staples) | |||
- 3 x timed filtered buffer discarded | |||
===Ran a 50 ml 2% Agarose-Gel with EtBr=== | |||
- 1kb ladder | |||
- p7560 | |||
- BM_2_1565 | |||
- BM_2_5Dh3ml | |||
- 1x filtered buffer | |||
- 2x filtered buffer | |||
Ask Marty about buffer and exact procedure!!! | |||
Revision as of 02:33, 6 September 2011
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Size exclusion filter purificationFolded samples BM_2_1565 (23.8.2011) and BM_2_5Dh3ml (23.8.2011) were filtered in the following manner- 50 µl from both samples were mixed and put into a Amicon filter - 400 µl buffer added - spinned for 5 minutes at 14000 rcf - 400 µl buffer added - spinned for 5 minutes at 14000 rcf - 400 µl buffer added - spinned for 5 minutes at 14000 rcf - turned the filter and sinned the purified sample into a new Epi for 2 minutes at 1000 rcf - 1 x and 2 x filtered buffer filled into separate Epi (the buffer, which came through the filter; if purification with spin columns works, this should only contain staples) - 3 x timed filtered buffer discarded Ran a 50 ml 2% Agarose-Gel with EtBr- 1kb ladder - p7560 - BM_2_1565 - BM_2_5Dh3ml - 1x filtered buffer - 2x filtered buffer Ask Marty about buffer and exact procedure!!!
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