Biomod/2011/TUM/TNT/LabbookA/2011/09/01: Difference between revisions
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==Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine== | ==Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine== | ||
* MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550. | * MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550. | ||
* The DNA intercalator | * The DNA intercalator ethidium bromide and the major groove binder Spermine are added at various concentrations accoring to their Kd-Values. | ||
* | * The Kd value of Spermin is 4,8µL and that of ethidium bromide is 12µL. | ||
* The concentrations of Spermin and ethidium bromide are as follows: | |||
Revision as of 03:38, 2 September 2011
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Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine
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