Biomod/2011/TUM/TNT/LabbookA/2011/08/30

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Measurement of DAPI concentration series for the helix MH255/256 in Real Time PCR

  • For description of preparation see 23.08.2011.
  • There was no significant connection between DAPI concentration and FRET efficiencies to be seen.
  • Probably, concentrations of fluorescent dyes were to low to be detected by Real Time PCR.

Preparation of samples for TEM

The major bands of the agarose gel from 29th August were prepared for observation in the TEM. The samples are:
BM1 2DH3ML, BM2 2DH3ML, BM1 5DH3ML, BM2 5DH3ML
sample preparation according to standard protocol
grids could not be observed, since TEM does not work at the moment

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