Biomod/2011/TUM/TNT/LabbookA/2011/08/17: Difference between revisions

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&epsilon;<sub>260</sub> (MH_256) = 1.59e5 M<sup>-1</sup>cm<sup>-1</sup> <br>
&epsilon;<sub>260</sub> (MH_256) = 1.59e5 M<sup>-1</sup>cm<sup>-1</sup> <br>
<br>
<br>
c (
c (MH_255 Atto 647N ddUTP) = 2.8µM <br>
c (MH_256 Atto 550 ddCTP = 7.1µM


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Revision as of 08:16, 5 September 2011

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determined absorption coefficients of MH_255 and MH_256 and concentrations of the labeled MH_255 Atto 647N ddUTP and MH_256 Atto 550ddCTP

according to standard procedures

results

ε260 (MH_255) = 1.58e5 M-1cm-1
ε260 (MH_256) = 1.59e5 M-1cm-1

c (MH_255 Atto 647N ddUTP) = 2.8µM
c (MH_256 Atto 550 ddCTP = 7.1µM