Biomod/2011/TUM/TNT/Home - Revision history

Florian Helmhold at 06:42, 3 November 2011

Florian Helmhold — Thu, 03 Nov 2011 06:42:10 GMT

←Older revision		Revision as of 06:42, 3 November 2011
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	Welcome to the TUM Biomod project 2011! On this page we will present a DNA origami which is used as a biosensor for DNA small molecule interactions. We would like to give you an overview about this project, how we got there, which difficulties we had to face and of course you also have the chance to get to know us a little bit.</br>		Welcome to the TUM Biomod project 2011! On this page we will present a DNA origami which is used as a biosensor for DNA small molecule interactions. We would like to give you an overview about this project, how we got there, which difficulties we had to face and of course you also have the chance to get to know us a little bit.</br>
	<br>		<br>
-	To find out more about <i>The U</i> we highly recommend ~~to watch~~ the video.	+	To find out more about <i>The U</i> we highly recommend watching the video.
	<br>		<br>
	<h1>Abstract</h1>		<h1>Abstract</h1>

Florian Helmhold at 06:41, 3 November 2011

Florian Helmhold — Thu, 03 Nov 2011 06:41:35 GMT

←Older revision		Revision as of 06:41, 3 November 2011
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	<div id="abstractandlinks">		<div id="abstractandlinks">
	<h1>Video</h1>		<h1>Video</h1>
-	<center><iframe width="800" height="450" src="http://www.youtube.com/embed/~~LPl1JDiLPQY~~" frameborder="0" allowfullscreen></iframe></center>	+	<center><iframe width="800" height="450" src="http://www.youtube.com/embed/cv4mFGpLC_w" frameborder="0" allowfullscreen></iframe></center>
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Alexander Hipp at 05:58, 3 November 2011

Alexander Hipp — Thu, 03 Nov 2011 05:58:50 GMT

←Older revision		Revision as of 05:58, 3 November 2011
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	<div id="abstractandlinks">		<div id="abstractandlinks">
	<h1>Video</h1>		<h1>Video</h1>
-	<center><iframe width="~~700~~" height="~~394~~" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>	+	<center><iframe width="800" height="450" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Alexander Hipp at 05:51, 3 November 2011

Alexander Hipp — Thu, 03 Nov 2011 05:51:46 GMT

←Older revision		Revision as of 05:51, 3 November 2011
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	<div id="abstractandlinks">		<div id="abstractandlinks">
	<h1>Video</h1>		<h1>Video</h1>
-	<center><iframe width="~~560~~" height="~~315~~" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>	+	<center><iframe width="700" height="394" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Simeon Leupold: Undo revision 563659 by Simeon Leupold (Talk)

Simeon Leupold — Thu, 03 Nov 2011 05:26:00 GMT

Undo revision 563659 by Simeon Leupold (Talk)

←Older revision		Revision as of 05:26, 3 November 2011
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	<h1>Video</h1>		<h1>Video</h1>
	<center><iframe width="560" height="315" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>		<center><iframe width="560" height="315" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>
-	~~<br>~~
-	~~<br>~~
-	~~<br>~~
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Simeon Leupold at 05:25, 3 November 2011

Simeon Leupold — Thu, 03 Nov 2011 05:25:14 GMT

←Older revision		Revision as of 05:25, 3 November 2011
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	<h1>Video</h1>		<h1>Video</h1>
	<center><iframe width="560" height="315" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>		<center><iframe width="560" height="315" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>
		+	<br>
		+	<br>
		+	<br>
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Alexander Hipp at 05:09, 3 November 2011

Alexander Hipp — Thu, 03 Nov 2011 05:09:22 GMT

←Older revision		Revision as of 05:09, 3 November 2011
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	<div id="abstractandlinks">		<div id="abstractandlinks">
	<h1>Video</h1>		<h1>Video</h1>
-	<center><iframe width="~~500~~" height="~~281~~" src="http://www.youtube.com/embed/~~A6rRGlAUx3k~~" frameborder="0" allowfullscreen></iframe></center>	+	<center><iframe width="560" height="315" src="http://www.youtube.com/embed/LPl1JDiLPQY" frameborder="0" allowfullscreen></iframe></center>
	</div>		</div>
	</body>		</body>
	</html>		</html>
	__TOC__		__TOC__

Frederik Schreiber at 03:21, 3 November 2011

Frederik Schreiber — Thu, 03 Nov 2011 03:21:00 GMT

←Older revision		Revision as of 03:21, 3 November 2011
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	<h1>Links</h1>		<h1>Links</h1>

-	<div id="side1" align="center"> <a href='#' id='lside1'></a></div>	+	<div id="side1" align="center"> <a href='http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Project' id='lside1'></a></div>
-	<div id="side2" align="center"> <a href='#' id='lside2'></a></div>	+	<div id="side2" align="center"> <a href='http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Results' id='lside2'></a></div>
-	<div id="side3" align="center"> <a href='#' id='lside3'></a></div>	+	<div id="side3" align="center"> <a href='http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods' id='lside3'></a></div>
-	<div id="side4" align="center"> <a href='#' id='lside4'></a></div>	+	<div id="side4" align="center"> <a href='http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Team' id='lside4'></a></div>
-	<div id="side5" align="center"> <a href='#' id='lside5'></a></div>	+	<div id="side5" align="center"> <a href='http://openwetware.org/wiki/Biomod/2011/TUM/TNT/LabbookA' id='lside5'></a></div>

Alexander Hipp at 00:17, 3 November 2011

Alexander Hipp — Thu, 03 Nov 2011 00:17:29 GMT

←Older revision		Revision as of 00:17, 3 November 2011
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	<body>		<body>
	<div id="abstractandlinks">		<div id="abstractandlinks">
		+	<div id="abstract">
	<h1>Welcome</h1>		<h1>Welcome</h1>
	Welcome to the TUM Biomod project 2011! On this page we will present a DNA origami which is used as a biosensor for DNA small molecule interactions. We would like to give you an overview about this project, how we got there, which difficulties we had to face and of course you also have the chance to get to know us a little bit.</br>		Welcome to the TUM Biomod project 2011! On this page we will present a DNA origami which is used as a biosensor for DNA small molecule interactions. We would like to give you an overview about this project, how we got there, which difficulties we had to face and of course you also have the chance to get to know us a little bit.</br>
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	Small DNA-binding molecules including intercalators and groove binders cause changes in the geometry of double-helical DNA which may affect transcriptional regulation and nuclease activity. Elucidating the structural changes imposed on DNA including changes in pitch and length can shed light on functional mechanisms in the case of compounds that show selective toxicity such as antibiotic and antiviral activity. Here we report on our efforts in developing a self-assembled DNA-Origami device that can be used both in a fluorometric assay and for transmission electron microscopy with the aim to classify whether a DNA binding molecule is an intercalator or groove binder, and that reports quantitatively on changes in pitch and length caused by DNA-binding molecules. We have tested our device with different DNA binders, spermine, ethidium bromide and DAPI, in different concentrations. In accordance with structure simulations, the local deformations caused by addition of these small molecules led to observable global structural changes.		Small DNA-binding molecules including intercalators and groove binders cause changes in the geometry of double-helical DNA which may affect transcriptional regulation and nuclease activity. Elucidating the structural changes imposed on DNA including changes in pitch and length can shed light on functional mechanisms in the case of compounds that show selective toxicity such as antibiotic and antiviral activity. Here we report on our efforts in developing a self-assembled DNA-Origami device that can be used both in a fluorometric assay and for transmission electron microscopy with the aim to classify whether a DNA binding molecule is an intercalator or groove binder, and that reports quantitatively on changes in pitch and length caused by DNA-binding molecules. We have tested our device with different DNA binders, spermine, ethidium bromide and DAPI, in different concentrations. In accordance with structure simulations, the local deformations caused by addition of these small molecules led to observable global structural changes.
	</div>		</div>
		+	<div id="links">
		+	<h1>Links</h1>

		+	<div id="side1" align="center"> <a href='#' id='lside1'></a></div>
		+	<div id="side2" align="center"> <a href='#' id='lside2'></a></div>
		+	<div id="side3" align="center"> <a href='#' id='lside3'></a></div>
		+	<div id="side4" align="center"> <a href='#' id='lside4'></a></div>
		+	<div id="side5" align="center"> <a href='#' id='lside5'></a></div>
		+
		+
		+
		+	</div>
		+	<div class="clear"></div>
	<div id="abstractandlinks">		<div id="abstractandlinks">
	<h1>Video</h1>		<h1>Video</h1>

Manuel Hora at 23:49, 2 November 2011

Manuel Hora — Wed, 02 Nov 2011 23:49:50 GMT

←Older revision		Revision as of 23:49, 2 November 2011
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	<br>		<br>

-	Small DNA-binding molecules including intercalators and groove binders cause changes in the geometry of double-helical DNA which may affect transcriptional regulation and nuclease activity. Elucidating the structural changes imposed on DNA including changes in pitch and length can shed light on functional mechanisms in the case of compounds that show selective toxicity such as antibiotic and antiviral activity. Here we report on our efforts in developing a self-assembled DNA-Origami device that can be used both in a fluorometric assay and for transmission electron microscopy with the aim to classify whether a DNA binding molecule is an intercalator or groove binder, and that reports quantitatively on changes in pitch and length caused by DNA-binding molecules. We have tested our device with different DNA binders, spermine, ethidium bromide and DAPI in different concentrations. In accordance with structure simulations, the local deformations caused by addition of these small molecules led to observable global structural changes.	+	Small DNA-binding molecules including intercalators and groove binders cause changes in the geometry of double-helical DNA which may affect transcriptional regulation and nuclease activity. Elucidating the structural changes imposed on DNA including changes in pitch and length can shed light on functional mechanisms in the case of compounds that show selective toxicity such as antibiotic and antiviral activity. Here we report on our efforts in developing a self-assembled DNA-Origami device that can be used both in a fluorometric assay and for transmission electron microscopy with the aim to classify whether a DNA binding molecule is an intercalator or groove binder, and that reports quantitatively on changes in pitch and length caused by DNA-binding molecules. We have tested our device with different DNA binders, spermine, ethidium bromide and DAPI, in different concentrations. In accordance with structure simulations, the local deformations caused by addition of these small molecules led to observable global structural changes.
	</div>		</div>