Biomod/2011/LMU/FolD'N'Assemble/Results
Summary
- The construct folded correctly.
- The cylinders dimerised correctly. The efficiency depends on the number of connections between the two cylinders
- The container openes at low pH depending on the salt concentration.
- The opening also would work in human cells because it works in PBS buffer.
Folding
As a first step, we designed the construct and looked for the best folding condition. We chose a cylindrical shape with a closed and an open end. [bild, cadnano file]
After some Experiments, we decided to use a MgCl2 concentration 0f 18 mM and an annealing time of 55 h. The pictures of the TEM show that the cylinders have the right shape and size. [tembild]
Dimerisation
We designed four different constructs: with 2,3,4 and 5 pairs of complementary strands. [bild]
More connection strands led to better dimerisation [gel]
Opening
To open the containers we did a buffer exchange.
We exchanged the original buffer with another buffer with low pH. For good results we needed to decrease the salt concentration.[bild]
To show that the opening process would work in the human cell, we used PBS buffer, which is isotonic to the human body.[bild]
