Biomod/2011/LMU/FolD'N'Assemble/Results

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THE PROJECT

RESULTS

PROTOCOLS

SCHEDULE

TEAM Timon Funck Aleksej Belizki Ralf Weidner Verena Schüller Prof. Tim Liedl

Summary

• The construct folded correctly.
• The cylinders dimerised correctly. The efficiency depends on the number of connections between the two cylinders
• The container openes at low pH depending on the salt concentration.

Folding

As a first step, we designed the construct and looked for the best folding condition. We chose a cylindrical shape with a closed and an open end. [bild, cadnano file]

After some Experiments, we decided to use a MgCl₂ concentration 0f 18 mM and an annealing time of 55 h. The pictures of the TEM show that the cylinders have the right shape and size. [tembild]

Dimerisation

We designed four different constructs: with 2,3,4 and 5 pairs of complementary strands. [bild]

More connection strands led to better dimerisation [gel]

Opening

To open the containers we did a buffer exchange.

We exchanged the original buffer with another buffer with low pH. For good results we needed to decrease the salt concentration.