Biomod/2011/LMU/FolD'N'Assemble/Results: Difference between revisions
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===Folding=== | ===Folding=== | ||
As a first step, we designed the construct and looked for the best folding | As a first step, we designed the construct and looked for the best folding conditions. | ||
We chose a cylindrical shape with a closed and an open end. [bild, cadnano file] | We chose a cylindrical shape with a closed and an open end. [bild, cadnano file] | ||
After some | After some experiments, we decided to use a MgCl<sub>2</sub> concentration of 18 mM and an annealing time of 55 h. | ||
The pictures of the TEM show that the cylinders have the right shape and size. [tembild] | The pictures of the TEM show that the cylinders have the right shape and size. [tembild] | ||
Revision as of 08:51, 31 October 2011
Summary
- The construct folded correctly.
- The cylinders dimerised correctly. The efficiency depends on the number of connections between the two cylinders
- The container openes at low pH depending on the salt concentration.
- The opening also would work in human cells because it works in PBS buffer.
Folding
As a first step, we designed the construct and looked for the best folding conditions. We chose a cylindrical shape with a closed and an open end. [bild, cadnano file]
After some experiments, we decided to use a MgCl2 concentration of 18 mM and an annealing time of 55 h. The pictures of the TEM show that the cylinders have the right shape and size. [tembild]
Dimerisation
We designed four different constructs: with 2,3,4 and 5 pairs of complementary strands. [bild]
More connection strands led to better dimerisation [gel]
Opening
To open the containers we did a buffer exchange.
We exchanged the original buffer with another buffer with low pH. For good results we needed to decrease the salt concentration.[bild]
To show that the opening process would work in the human cell, we used PBS buffer, which is isotonic to the human body.[bild]
