Biomod/2011/LMU/FolD'N'Assemble/Results: Difference between revisions
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===Dimerisation=== | ===Dimerisation=== | ||
We designed four different constructs: with 2,3,4 and 5 pairs of complementary strands. [bild] | |||
More connection strands led to better dimerisation [gel] | |||
===Opening=== | ===Opening=== | ||
Revision as of 05:18, 31 October 2011
Summary
- The construct folded correctly.
- The cylinders dimerised correctly. The efficiency depends on the number of connections between the two cylinders
- The container openes at low pH depending on the salt concentration.
Folding
As a first step, we designed the construct and looked for the best folding condition. We chose a cylindrical shape with a closed and an open end. [bild, cadnano file]
After some Experiments, we decided to use a MgCl2 concentration 0f 18 mM and an annealing time of 55 h. The pictures of the TEM show that the cylinders have the right shape and size. [tembild]
Dimerisation
We designed four different constructs: with 2,3,4 and 5 pairs of complementary strands. [bild]
More connection strands led to better dimerisation [gel]
