Biomod/2011/Harvard/HarvarDNAnos:Results AuNP: Difference between revisions

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==Incorporation with our DNA Origami Structures==
==Incorporation with Our DNA Origami Structures==
* With respect to incorporating our gold nanoparticles into our DNA Origami, we were only successful when we used gold nanoparticles that we had ordered commercially, but that we had conjugated with our DNA strands of choice.
* With respect to incorporating our gold nanoparticles into our DNA Origami, we were only successful when we used gold nanoparticles that we had ordered commercially, but that we had conjugated with our DNA strands of choice.
* See:  ''[[Biomod/2011/Harvard/HarvarDNAnos:Results_Box | Box Results]]'' for a description of our results
* See ''[[Biomod/2011/Harvard/HarvarDNAnos:Results_Box | Box Results]]'' for a description of our results.
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Revision as of 07:34, 2 November 2011

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Gold Nanoparticles Results

...Return to Results

Synthesis

In general, gold nanoparticles can be either synthesized or ordered commercially. To see how we synthesized our own particles, see here.

5-nanometer particle analysis

  • 5-nm gold nanoparticles ordered commercially (2 months old); there is aggregation in this sample

  • 5-nm gold nanoparticles that we synthesized


Larger particle analysis

Below are DLS outputs, which show that the more gold particles from a stock solution of 15nm particles introduced to a given volume of AuCl, the smaller the gold aggregates that form (more particles = wider distribution of the aqueous gold)

  • Hydrodynamic Radius of Particles after Addition of 500 uL of 15nm Au Particle Stock Solution

  • Hydrodynamic Radius of Particles after Addition of 200 uL of 15nm Au Particle Stock Solution

  • Hydrodynamic Radius of Particles after Addition of 50 uL of 15nm Au Particle Stock Solution

  • Hydrodynamic Radius of Particles after Addition of 20 uL of 15nm Au Particle Stock Solution

  • Hydrodynamic Radius of Particles after Addition of 5 uL of 15nm Au Particle Stock Solution


Phosphene Modification

The above files are links to the DLS measurements of my gold particles synthesized with a target diameter of 5nm before adding phosphene. The first measurement shows a uniform distribution of particles upon initial synthesis (with a hydrodynamic radius of about 12 nm). The second document, "PrePhosphene_4DaysLater" shows that there was a degree of aggregation of AuNP after sitting at 4 degrees C for four days without the addition of phosphene.


Size Analysis using TEM and ImageJ

Using ImageJ and TEM, we can determine the average size of our particles. See here for more info.

  • TEM Images of our synthesized particles

  • Histogram showing the size distribution of our synthesized particles, with an average diameter of 6.61nm


Conjugating DNA to Our Gold Nanoparticles

For a description of the conjugation protocol we followed, see here.

  • Above is an image depicting clear banding of our AuNP-DNA conjugates, indicating the success of conjugation. Each band contains gold nanoparticles with a distinct number of attached DNA strands. We were able to perform this procedure both with gold nanoparticles we synthesized on our own, and with gold nanoparticles that we ordered commercially.


Incorporation with Our DNA Origami Structures

  • With respect to incorporating our gold nanoparticles into our DNA Origami, we were only successful when we used gold nanoparticles that we had ordered commercially, but that we had conjugated with our DNA strands of choice.
  • See Box Results for a description of our results.


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