Biomod/2011/Harvard/HarvarDNAnos:Results
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Results Overview
We have accomplished the following:
- With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.
- We have opened Han’s sphere in preparation for future nanocargo encapsulation.
- We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.
Box Container
- We designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
- We demonstrated that we can close and open the box with high efficiency.
- We demonstrated that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
- We demonstrated that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.
See also: Rectangular Box Design, Rectangular Box Methods
Spherical Container
With our sphere design, we were able to:
- Fold and characterize through atomic force microscopy (AFM) the original Han et al. sphere, which we call the "closed" sphere.
- Fold and characterize through AFM our "open" sphere in which we removed all equator staple strands.
- Test various lock mechanisms to transition between the closed and open states of the sphere.
- Analyze the scaffold-staple-lock system to find out why certain lock designs were unsuccessful at closing the sphere.
See also: Sphere Design, Sphere Methods
Nanoparticles
- We synthesized 5nm gold particles and conjugated DNA to their surfaces
- We conjugated DNA to commercially available 5 nm gold nanoparticles
- We integrated AuNP-DNA conjugates into our rectangular box design
Continue reading...
See also: Cargo Design
Photo-cleavage
- We demonstrated photo-cleavage of strands with internal photo-cleavable spacers
- We outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo
Continue reading...
See also: Cargo Design
Tools and Parts
