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| {{Template:Biomod/2011/Harvard/HarvardDNAnos}} | | {{Template:Biomod/2011/Harvard/HarvardDNAnos}} |
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| <div style="padding: 10px; width: 800px; border: 5px solid #333333;"> | | =Process= |
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| [[Image:Project Outline Header.jpg | center]]
| | <a href=http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Designs><img src="http://openwetware.org/images/3/3c/Design.jpg" width=150px></a> |
| | | <a href=http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Methods><img src="http://openwetware.org/images/a/ac/Methods.jpg" width=150px></a> |
| | | <a href=http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Results><img src=http://openwetware.org/images/4/47/Hdna_results.jpg width=150px></a> |
| | | <a href=http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Protocols><img src="http://openwetware.org/images/3/3a/Protocols.jpg" width=150px></a> |
| ==Objective== | | <a href=http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Tutorials><img src="http://openwetware.org/images/2/24/Tutorials.jpg" width=150px></a> |
| Our goal is to create DNA origami containers that can load, hold, and release cargo. To do this, we must:
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| *Design and fold robust [[#Container Designs | three dimensional origami featuring enclosed interiors]] with optimized volumes
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| *Design and implement [[#Opening/Closing and Loading/Solubilization Mechanisms | opening/closing mechanisms and loading/solubilization mechanisms]] for our containers that allow us to controllably:
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| :#Load various forms of cargo by attaching it to the inside of a container and then closing the container | | ==Lab Notebooks== |
| :#Solubilize this cargo without leakage to the exterior of the container
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| :#Open our container, releasing our cargo
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| | | <a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:LabNotebook_Nick"><img src="http://openwetware.org/images/7/73/Notebook_nick.jpg" width=150px></a> |
| ==Container Designs== | | <a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:LabNotebook_Sherrie"><img src="http://openwetware.org/images/e/ed/Notebook_sherrie.jpg" width=150px></a> |
| | | <a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:LabNotebook_Evan"><img src="http://openwetware.org/images/8/86/Notebook_evan.jpg" width=150px></a> |
| | | <a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:LabNotebook_Shwinn"><img src="http://openwetware.org/images/d/de/Notebook_shwinn.jpg" width=150px></a> |
| ===Sphere=== | | </center> |
| *Using caDNAno, we have [[Biomod/2011/Harvard/HarvarDNAnos:Documents#Spherical Container Design | replicated the sphere]] featured in the [http://www.sciencemag.org/content/332/6027/342.full recent Science paper by Han et al.] so that we can generate the exact same staple sequences published by Han et al.
| | </html> |
| *Now we need to implement [[#Opening/Closing and Loading/Solubilization Mechanisms | opening/closing and loading/solubilization mechanisms]] for the sphere
| | ==[[Biomod/2011/Harvard/HarvarDNAnos:Presentations | Presentations]]== |
| *We also need to cap the holes (approximately 2-3 nm in diameter) at the poles of the sphere, or figure out how to exploit these holes
| | ==[[Biomod/2011/Harvard/HarvarDNAnos:caDNAnoFiles | Repository of caDNAno Files]]== |
| *We are in the process of creating "SphereCAD," a computer program that synthesizes our caDNAno file and 3D models in order to streamline correlation between theta/phi, bp position, and whether that bp position points into or out of the sphere. This will help in the design process of O/C and L/S mechanisms.
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| :[[Image:Sphere_Image.jpeg | 300x300px]] | |
| :3D model of our sphere, created in Autodesk Maya
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| ===Box with Lid===
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| *We have designed a [[Biomod/2011/Harvard/HarvarDNAnos:Documents#Box Container Design | box with double layer walls, lid with perpendicular helices and a double hinge]]
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| **We have designed one [[Biomod/2011/Harvard/HarvarDNAnos:Documents#2011-06-17 | O/C mechanism for this box]], which utilizes parallel locking handles and an [[#Azobenzene | azobenzene strand]] or azobenzene-sequence-with-toehold lock
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| ::[[Image:Perkons8.jpg | 300x300px]]
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| ::Schematic of Box with Lid with Parallel Locking Mechanism, to be locked by azobenzene-sequence strand
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| :*This design's strands have been ordered
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| *We now should implement [[Biomod/2011/Harvard/HarvarDNAnos:LabNotebook Evan#Day 2 (2011-06-07) | compression (see second image)]] so that extra force must be applied in order to tightly seal the box, minimizing fluctuations of the lid
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| *We should also explore other [[#Opening/Closing and Loading/Solubilization Mechanisms | O/C mechanisms]]
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| [[Image:2011-06-20_figure1.jpg | 500px]]
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| *We need to implement a [[#Opening/Closing and Loading/Solubilization Mechanisms | L/S mechanism]]
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| *We should also consider designing a box that has a lid with parallel helices so that the jagged interface between box and lid (due to crossover positions) will be complementary
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| ==Other Discussed Designs==
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| ===Torus Design===
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| *We want to explore the idea of working around weakness of sphere design (two holes at poles) by creating the same orientation of rings and additionally extending an inner layer that connects the top and bottom of the sphere to create the "no holes" torus
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| *Potential ideas:
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| **Thiolated strands within the "inner" layer of rings
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| **Strands exist as cross-linked disulfide bridges in natural confirmation
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| **Upon exposure to reducing environment, disulfide bridges cleaved to temporarily create small crevice within torus for nanoparticle uptake
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| **Time-sensitive re-oxidation to close torus
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| ===Concentric Rings===
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| *We have [[Biomod/2011/Harvard/HarvarDNAnos:Documents#2011-06-10 | replicated in caDNAno]] the Nine Concentric Rings structure in Han et al
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| ===Ellipsoid===
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| *received caDNAno file from Han
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| ===Vase===
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| *received caDNAno file from Han
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| ==Opening/Closing and Loading/Solubilization Mechanisms==
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| ==="One-pot" folding vs. Fold-then-load===
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| *
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| *
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| ===Azobenzene===
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| [[Image:Liang azobenzene.png | 500px]]
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| [http://onlinelibrary.wiley.com/doi/10.1002/cbic.200700649/full Liang et al (2008)]
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| *First order of strands for [[#Box with Lid | Box with Lid]] has lock handles adapted to azobenzene sequence. Two options:
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| **use actual azobenzene strand to lock, UV to unlock
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| **use azobenzene-sequence strand with toehold to lock, strand displacement to unlock
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| *We will first test both mechanisms on a [[Biomod/2011/Harvard/HarvarDNAnos:Documents#2011-06-17_2 | "minimal box"]] which is just a strand of DNA with a central poly T representing the actual box
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| ===Strand Displacement===
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| [[Image:2011-06-20_figure3.jpg | 500px]]
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| Disadvantages
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| *Single strands probably diffuse slowly into sphere
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| ===Disulfide Linkers===
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| [[Image:5' 3' disulfide formation.png | 500px]]
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| :How to Create Disulfide-Linked Staples
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| [[Image:Disulfide2.jpg | 500px]]
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| :Disulfide Linkers as a Mechanism to Open the Sphere
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| Advantages:
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| *cleaved by DTT or TCEP, which are small molecules that would quickly diffuse into the sphere
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| [[Image:Disulfide linker for solubilization revised.png | 500px]]
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| Experiments:
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| *We are currently testing our ability to create disulfide bonds by just using two 5'-thiolated strands
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| *We are trying to find the optimal pH for oxidation by using a pH reaction ladder and trying to determine if bringing pH back down will reverse the reaction
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| *We also want to test the cleavage of the disulfide bond with DTT
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| *We will analyze this system with PAGE
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| [[Image:5' 5' disulfide formation.png | 500px]]
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| * NOTE: As of July 1st, we are no longer pursuing the use of disulfide linkers within our structures due to the nature of the cargo we wish to contain within our origami
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| ===Restriction Enzymes===
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| Advantages
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| * Precise cut locations
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| * m13mp18 does not contain the cut sequence of the NOT1 Restriction Enzyme
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| Disadvantages
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| * Not sure if this is entirely compatible with DNA origami, but have designed a mechanism outlined below to see if such a configuration will work well
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| * Not sure of how long pre and post-cut excesses should be
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| [[Image:Perkons9.jpg | 400x700px]]
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| :Mechanism for testing if restriction enzymes are compatible with origami structures
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| [[Image:RestrictionEnzymeModel2.jpg | 400x700px]]
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| :Restriction Enzyme Model for Opening a Sphere
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| ===Photocleavable Spacers===
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| [[Image:Sphere_pcspacer.jpg | 500px]]
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| [http://www.idtdna.com/catalog/Modifications/Modifications.aspx?catid=6 From IDT]
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| ===Hetero-bifunctional crosslinkers that convert internal amine-modified bases to thiols===
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| *
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| *
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| ===Cargo Experimentation===
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| * Coming soon!
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| ====Making chains of nanoparticles using "ultramer"====
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| [[Image:Nanoparticle Chain.png | 500px]]
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| *We are currently working on attaching the handles to the ultramer
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| **We ran a [[Biomod/2011/Harvard/HarvarDNAnos:LabNotebook Evan#Day 12 (2011-06-21)| reaction ladder]] where the first reaction had only the ultramer, the second had the ultramer with the first handle annealed, the second had the ultramer, first handle, and second handle, etc. Here is the [[Biomod/2011/Harvard/HarvarDNAnos:Documents#2011-06-23 | PAGE result]].
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| *Next, we need to figure out how to attach thiolated DNA strands to gold nanoparticles
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| ====Synthesizing nanoparticles====
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| *We have made our [[Biomod/2011/Harvard/HarvarDNAnos:LabNotebook Evan#Day 13 (2011-06-22) | own homemade gold nanoparticles]], 5 nm (as per "Preparing Colloidal Gold for Electron Microscopy" by Polysciences, Inc.) and other sizes
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| *We have used [[Biomod/2011/Harvard/HarvarDNAnos:Documents#Making Gold Nanoparticles | DLS to confirm their size]] and determine their hydrodynamic radius
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| *Next, we will use TEM to image these nanoparticles, varying salt conditions to observe aggregation
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| ====Using small origami as cargo====
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| *triangle
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| *nanoball
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| *ring
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| ==Experimental Permutations of Containers to Test==
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| ===Sphere Design===
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| # Normal sphere
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| # Sphere with disulfide on equator and cargo handle staples
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| # Sphere with strand displacement cargo handle staples, normal strands on equator --> tells us if single strands diffuse into sphere through top and bottom holes
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| # Sphere with strand displacement equator and cargo handle staples
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| # Sphere with strand displacement cargo handle staples, disulfide on equator (?)
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| # Sphere with strand displacement equator staples, disulfide handle staples (?)
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| # Sphere with restriction enzyme equator staples, disulfide cargo handle staples
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| # Sphere with azobenzene staples
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| *NOTE 1: If from (3) we see that single strands do diffuse into the sphere, in the next round of design we can add capping mechanisms to seal off the top and bottom holes of the sphere. (Maybe even create a torus!)
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| *NOTE 2: To test our program, we eventually need to attach cargo to outside.
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| ===Box Design===
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| *Bare box without cargo L/S mechanism
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| **actual azobenzene: F_12X (folds open, lock, then UV release)
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| **azobenzene sequence with toehold: F_n+ (folds open, lock, then strand displacement release)
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| **intra-molecular locking (folds locked, then strand displacement release)
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| *Box with cargo L/S mechanism
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| ==Staple Strands to Order by Origami Structure==
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| ===Sphere Design===
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| #Matching strands on equator
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| #Matching strands not on equator
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| #Non-matching strands on equator (from Han's paper)
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| #Matching on equator (from Han's paper)
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| #Non-matching strands on equator (from our caDNAno file)
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| :*Staples with disulfide
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| :*Staples with strand displacement mechanism
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| :*Staples with restriction enzyme sequences
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| :*Staples with azobenzene
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| #Matching on equator (from our caDNAno file)
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| ===Box Design===
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| #Core staples to not be manipulated
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| #Lock staples
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| #Staples that might be affected when direction of lock staples is changed
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| #Staples from which cargo-binding mechanism may be attached
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| </div>
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| <html> | | <html> |
