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=Motivation=
=Motivation=
[[Image:Yellow-submarine-icon copy.jpg |thumb|right|Figure 1. "Nano-submarine"]]
[[Image:Yellow-submarine-icon copy.jpg|right|thumb|Figure 1. "Nano-submarine"]]
[[Image:Dynamite.jpg |thumb|right|Figure 2. Simultaneous detection and amplification]]
[[Image:Dynamite.jpg |thumb|right|Figure 2. Simultaneous detection and amplification]]
At the beginning of summer, there were two ideas that particularly excited us (and continue to excite us today):  
At the beginning of summer, two ideas particularly excited us:  
# the development of "nano-submarines" (Figure 1) that could programmably deliver and release drugs to, for example, specific parts of the body or specific parts of the cell, and  
# the development of "nano-submarines" (Figure 1) that could '''programmably deliver and release drugs''' to, for example, specific parts of the body or specific parts of the cell, and  
# the realization of a one-step detection and amplification system (Figure 2) that could be deployed to augment the efficiency of catalytic DNA circuits or simplify the assay of molecular or heavy metal signals.  
# the realization of a '''one-component detection and amplification''' system (Figure 2) that could be deployed to augment the efficiency of catalytic DNA circuits or simplify the assay of molecular or heavy metal signals.  
Here is a record of our early [[Biomod/2011/Harvard/HarvarDNAnos:Brainstorming|brainstorming]].
Here is a record of our early [[Biomod/2011/Harvard/HarvarDNAnos:Brainstorming|brainstorming]].


=Inspiration=
=Inspiration=
[[Image:Smileys.png |thumb|right|150px|Figure 3. Rothemund (2006)]]
[[Image:Han et al.png |thumb|right|150px|Figure 4. Han et al. (2011): DNA origami spheres]]
[[Image:Andersen.png |thumb|right|150px|Figure 5. Andersen et al. (2009): DNA origami box that can open and close]]
The elegance and robustness of '''scaffolded DNA origami technology''' (Figure 3) inspired us, especially after our literature review revealed that other researchers had already used the origami technique to create three-dimensional nano-structures with enclosed interiors.
*[http://www.sciencemag.org/content/332/6027/342.full Han's development of spherical origami] (Figure 4) appealed to us because of its '''efficient use of DNA''' and the inherent lack of "weak points" in spheres.
*[http://www.nature.com/nature/journal/v459/n7243/full/nature07971.html Andersen's box] (Figure 5) also fascinated us because it  demonstrated the '''ability to open and close'''.
We realized that further functionalizing these types of nano-structures so that they could '''controllably load, entrap, and then later release cargo''' would bring us closer to the realization of both of the ideas that originally motivated us.
*Essentially we wanted to build upon Andersen's paper, in which he notes the potential for "the controlled release of nanocargos," and that "the DNA box presented here has the potential to both sense and act, for example by combining a diagnostic sensor of complex signals with the controlled release of, or access to, a payload."
=Goals=
'''Our overall goal was to create DNA origami containers that can load, hold, and release cargo.'''
Specifically, we aimed to:
*Design and fold robust [[Biomod/2011/Harvard/HarvarDNAnos:Design | three dimensional origami featuring enclosed interiors]] with optimized volumes
*Design and implement '''opening/closing mechanisms''' and '''loading/solubilization mechanisms''' for our containers that allow us to controllably:
#load various forms of cargo by attaching it to the inside of a container and then closing the container,
#solubilize this cargo without leakage to the exterior of the container, and
#open our container, releasing our cargo.


The elegance and robustness of scaffolded DNA origami technology (Figure 3) inspired us, especially after our literature review revealed that other researchers had already used the origami technique to create three-dimensional nano-structures with enclosed interiors. For example, [http://www.sciencemag.org/content/332/6027/342.full Han et al.'s development of spherical origami] (Figure 4) appealed to us because of its efficient use of DNA and the inherent lack of "weak points" in spheres. [http://www.nature.com/nature/journal/v459/n7243/full/nature07971.html Andersen et al.'s box] (Figure 5) also fascinated us because he already demonstrated the box's ability to open and close.


We realized that further functionalizing these types of nano-structures so that they could controllably load, entrap, and then later release cargo would bring us closer to the realization of both of the ideas that originally motivated us. Essentially we wanted to build upon Andersen's paper, in which he notes the potential for "the controlled release of nanocargos," and that "the DNA box presented here has the potential to both sense and act, for example by combining a diagnostic sensor of complex signals with the controlled release of, or access to, a payload."
[[Image:Smileys.png |thumb|left|150px|Figure 3. Rothemund (2006)]]
[[Image:Han et al.png |thumb|center|150px|Figure 4. Han et al. (2011): DNA origami spheres]]
[[Image:Andersen.png |thumb|right|150px|Figure 5. Andersen et al. (2011): DNA origami box that can open and close]]


=Goals=
Therefore, our goal is to create DNA origami containers that can load, hold, and release cargo. To do this, we aim to:


*Design and fold robust [[Biomod/2011/Harvard/HarvarDNAnos:Design | three dimensional origami featuring enclosed interiors]] with optimized volumes
*Design and implement opening/closing mechanisms and loading/solubilization mechanisms for our containers that allow us to controllably:
:#Load various forms of cargo by attaching it to the inside of a container and then closing the container
:#Solubilize this cargo without leakage to the exterior of the container
:#Open our container, releasing our cargo




Latest revision as of 15:08, 2 November 2011

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Home              Mission              Process              Designs              Results              Resources              Team

Motivation

Figure 1. "Nano-submarine"
Figure 2. Simultaneous detection and amplification

At the beginning of summer, two ideas particularly excited us:

  1. the development of "nano-submarines" (Figure 1) that could programmably deliver and release drugs to, for example, specific parts of the body or specific parts of the cell, and
  2. the realization of a one-component detection and amplification system (Figure 2) that could be deployed to augment the efficiency of catalytic DNA circuits or simplify the assay of molecular or heavy metal signals.

Here is a record of our early brainstorming.

Inspiration

Figure 3. Rothemund (2006)
Figure 4. Han et al. (2011): DNA origami spheres
Figure 5. Andersen et al. (2009): DNA origami box that can open and close

The elegance and robustness of scaffolded DNA origami technology (Figure 3) inspired us, especially after our literature review revealed that other researchers had already used the origami technique to create three-dimensional nano-structures with enclosed interiors.

We realized that further functionalizing these types of nano-structures so that they could controllably load, entrap, and then later release cargo would bring us closer to the realization of both of the ideas that originally motivated us.

  • Essentially we wanted to build upon Andersen's paper, in which he notes the potential for "the controlled release of nanocargos," and that "the DNA box presented here has the potential to both sense and act, for example by combining a diagnostic sensor of complex signals with the controlled release of, or access to, a payload."

Goals

Our overall goal was to create DNA origami containers that can load, hold, and release cargo. Specifically, we aimed to:

  1. load various forms of cargo by attaching it to the inside of a container and then closing the container,
  2. solubilize this cargo without leakage to the exterior of the container, and
  3. open our container, releasing our cargo.









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