Biomod/2011/Harvard/HarvarDNAnos:Designs

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Home              Mission              Process              Designs              Results              Resources              Team

Rectangular Box Container Design

Figure 1. A CanDo model of a component of our rectangular box.

Andersen's box impressed us with its ability to open and close, but we worried about its robustness and tightness as a container.

  • Cryo-EM imaging performed by Andersen revealed that the faces are either bent inward or outward.
  • Furthermore, the Andersen box is formed from a one-layer DNA sheet and, as such, is held together by five potentially weak seams.

Therefore, with the help of Wei Sun, we have designed our own box, which we feel stands a much better chance of keeping cargo inside and which is more straightforward to fold and to characterize.

Continue reading...

See also: Rectangular Box Results, Rectangular Box Methods

Spherical Container Design

Figure 2. A three-dimensional model of the Han sphere (Han et al. 2011).

In our search for a robust and elegant design, we were inspired by the origami sphere that Dongran Han demonstrated in his 2011 Science paper "DNA Origami with Complex Curvatures in Three-Dimensional Space" (Figure 1).

The spherical design appealed to us because of its efficient use of DNA and lack of weak points--that is, instead of having edges, it only has two holes at each pole, minimizing spots where cargo can leak out. We imagined that we would be able to change the design of the Han sphere to make it an openable and closable container.

Continue reading...

See also: Sphere Results, Sphere Methods

Cargo

With a few container designs in mind, our next goal was to provide them with functionality.

  • We decided to use 5 nm gold nanoparticle cargo as a test platform for our ability to capture, contain, and controllably release cargo.
  • We decided to use 5 nm gold nanoparticles because the sharp contrast they provide under TEM would help us to classify our results easily.
Figure 3. We conjugated ssDNA strands to 5 nm gold nanoparticles.

Continue reading...

See also: Cargo in the Sphere, Cargo in the Box, Nanoparticle Results, Photocleavage Results



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