Biomod/2011/Harvard/HarvarDNAnos: Difference between revisions

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{{Template:Biomod/2011/Harvard/HarvardDNAnos}}
{{Template:Biomod/2011/Harvard/HarvardDNAnos}}
<html><a href= http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos:Results><img src = http://openwetware.org/images/a/ac/Hdna_2011_logo1.png width=800px></a></html>
=News=
'''2012/02/16''': We are now recruiting for next year's Harvard BIOMOD team! Please contact Mingjie.Dai@wyss.harvard.edu if you are interested!
'''2011/11/05''': We won '''3rd Place''' at [http://biomod.net/ The International Bio-molecular Design Competition]! Please also check out the [[Biomod/2011 | websites of all of our incredible competitors]]. Congrats to everyone!


<html>
=DNA Origami Containers=
<center><iframe width="560" height="315" src="http://www.youtube.com/embed/7gjLVt9onsw" frameborder="0" allowfullscreen></iframe></center>
Previous work has showcased the use of scaffolded DNA origami to self-assemble nanoscale, three-dimensional shapes with enclosed, hollow interiors. '''We explore the possibility of using such structures to load, entrap, and release soluble nanoscale cargo''', with potential future applications in drug delivery and molecular signal amplification. Specifically, we design a rectangular box structure that can encapsulate gold nanoparticles and subsequently release them by a two-step process: a) photocleavage, which solubilizes the nanoparticle within the box, and b) introduction of a DNA signal, which opens the box by strand-displacement. We further investigate the use of an existing spherical origami design for similar purposes, and demonstrate opening of the sphere.
</html>
 
Previous work has showcased the use of scaffolded DNA origami to self-assemble nanoscale, three-dimensional shapes with enclosed, hollow interiors. We explore the possibility of using such structures to load, entrap, and release soluble nanoscale cargo, with potential future applications in drug delivery and molecular signal amplification. Specifically, we design a rectangular box structure that can encapsulate gold nanoparticles and subsequently release them by a two-step process: a) photocleavage, which solubilizes the nanoparticle within the box, and b) introduction of a DNA signal, which opens the box by strand-displacement. We further investigate the use of an existing spherical origami design for similar purposes, and demonstrate opening of the sphere.
 
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</a></center></marquee>
</html>
 
 
{|cellspacing="0"
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==Project==
===Overview===
*[[Biomod/2011/Harvard/HarvarDNAnos:Project | Project Outline]]
*[[Biomod/2011/Harvard/HarvarDNAnos:Brainstorming | Brainstorming]]
 
===Methods===
*[[Biomod/2011/Harvard/HarvarDNAnos:Protocols | Protocols]]
*[[Biomod/2011/Harvard/HarvarDNAnos:Tutorials | Tutorials]]
*[[Biomod/2011/Harvard/HarvarDNAnos:Lab_Notebook | Lab Notebook]]


===Output===
=Presentation=
*[[Biomod/2011/Harvard/HarvarDNAnos:Results | Results]]
<html><center>
*[[Biomod/2011/Harvard/HarvarDNAnos:Data&Output | Data]]
<div class="prezi-player"><style type="text/css" media="screen">.prezi-player { width: 550px; } .prezi-player-links { text-align: center; }</style><object id="prezi_uj6xu0za4jti" name="prezi_uj6xu0za4jti" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" width="550" height="400"><param name="movie" value="http://prezi.com/bin/preziloader.swf"/><param name="allowfullscreen" value="true"/><param name="allowscriptaccess" value="always"/><param name="bgcolor" value="#ffffff"/><param name="flashvars" value="prezi_id=uj6xu0za4jti&amp;lock_to_path=1&amp;color=ffffff&amp;autoplay=no&amp;autohide_ctrls=0"/><embed id="preziEmbed_uj6xu0za4jti" name="preziEmbed_uj6xu0za4jti" src="http://prezi.com/bin/preziloader.swf" type="application/x-shockwave-flash" allowfullscreen="true" allowscriptaccess="always" width="550" height="400" bgcolor="#ffffff" flashvars="prezi_id=uj6xu0za4jti&amp;lock_to_path=1&amp;color=ffffff&amp;autoplay=no&amp;autohide_ctrls=0"></embed></object><div class="prezi-player-links"></div></div>
*[[Biomod/2011/Harvard/HarvarDNAnos:SphereCAD | SphereCAD]]
</center></html>
*[[Biomod/2011/Harvard/HarvarDNAnos:caDNAnoFiles | Repository of caDNAno Files]]


|width="300" style="border: 0px solid #aaaaaa; color: #000; background-color: white"|
=Video=
<div style="clear: right; text-align: left; float: left; padding: .1em 1.5em .9em">
<center>
<html><iframe width="560" height="315" src="http://www.youtube.com/embed/NaAE_4AFVlA" frameborder="0" allowfullscreen></iframe></html>
</center>


==Team==
=Thank you=
 
<center>
===Undergraduate Team Members===
<html><a href = 'http://www.biomod.net'><img src= http://openwetware.org/images/0/0c/Biomod_logo.png width=220px> </a></html>
*[[User:Evan Wu |Evan Wu]]
<html><a href = 'http://www.wyss.harvard.edu'><img src= http://openwetware.org/images/f/f1/Wyss_logo.gif width=220px> </a></html>
*[[User:Sherrie Wang  |Sherrie Wang]]
<html><a href = 'http://www.hhmi.org'><img src= http://openwetware.org/images/a/ae/Hhmi2.jpg width=220px> </a></html>
*[[User:Nick Perkons |Nick Perkons]]
<html><a href = 'http://www.harvard.edu'><img src= http://openwetware.org/images/9/9c/Harvard_shield_wreath.png width=100px> </a></html>
*[[User:Shwinn Ricci |Shwinn Ricci]]
</center>
 
</div>
===Graduate and Post-Doctoral Mentors===
*[[User:Adam Marblestone |Adam Marblestone]]
*[[User:Ralf Jungmann |Ralf Jungmann]]
*[http://www.linkedin.com/profile/view?id=90655500&locale=en_US&trk=tab_pro Steve Perrault]
*[[User:Tom Schaus |Tom Schaus]]
*[http://yin.hms.harvard.edu/people/sun.wei/index.html Wei Sun]
*[http://www.dna.caltech.edu/~dzhang/ Dave Zhang]
 
===Faculty Mentors===
*[http://research4.dfci.harvard.edu/shih/SHIH_LAB/Home.htm William Shih]
*[http://yin.hms.harvard.edu/ Peng Yin]
 
 
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<div style="clear: right; text-align: left; float: left; padding: .1em 1.5em .9em">
 
==Resources==
 
===[[Biomod/2011/Harvard/HarvarDNAnos:Literature | <font color="#000000">Literature</font>]]===
 
===Applications & Tools===
*[http://cadnano.org/ caDNAno]
*[http://cando.dna-origami.org/ CanDo]
*[http://usa.autodesk.com/maya/ Maya 3D Design]
*[http://success.adobe.com/en/na/sem/products/illustrator.html?kw=p&sdid=FNRPC&skwcid=TC|22174|adobe%20illustrator||S|b|7724724036 Adobe Illustrator]
*[http://www.photoshop.com/ Adobe Photoshop]
*[http://greengenes.lbl.gov/cgi-bin/nph-massage.cgi Nucleic Acid Sequence Massager]
*[http://j5.jbei.org/VectorEditor/VectorEditor.html J5 Vector Editor]
*[http://www.nupack.org NuPack]
*[http://www.wolframalpha.com WolframAlpha]
*[http://www.xps2pdf.org/ XPS 2 PDF]
*[http://office.microsoft.com/en-us/excel/ Microsoft Excel]
*[http://excel2wiki.net/ Shawn Douglas' Excel-to-Wiki Converter]
 
===BioMod & OWW===
*[http://www.biomod.net BioMod]
*[http://wyss.harvard.edu/ The Wyss Institute]
*[http://www.harvard.edu/ Harvard University]
*[http://www.hhmi.org/ Howard Hughes Medical Institute]
*[[Etiquette|OpenWetWare Etiquette]]
 
<br><br><br><br><br><br><br><br>
 
 
|}


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Home              Mission              Process              Designs              Results              Resources              Team

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News

2012/02/16: We are now recruiting for next year's Harvard BIOMOD team! Please contact Mingjie.Dai@wyss.harvard.edu if you are interested! 2011/11/05: We won 3rd Place at The International Bio-molecular Design Competition! Please also check out the websites of all of our incredible competitors. Congrats to everyone!

DNA Origami Containers

Previous work has showcased the use of scaffolded DNA origami to self-assemble nanoscale, three-dimensional shapes with enclosed, hollow interiors. We explore the possibility of using such structures to load, entrap, and release soluble nanoscale cargo, with potential future applications in drug delivery and molecular signal amplification. Specifically, we design a rectangular box structure that can encapsulate gold nanoparticles and subsequently release them by a two-step process: a) photocleavage, which solubilizes the nanoparticle within the box, and b) introduction of a DNA signal, which opens the box by strand-displacement. We further investigate the use of an existing spherical origami design for similar purposes, and demonstrate opening of the sphere.

Presentation

<html><center> <div class="prezi-player"><style type="text/css" media="screen">.prezi-player { width: 550px; } .prezi-player-links { text-align: center; }</style><object id="prezi_uj6xu0za4jti" name="prezi_uj6xu0za4jti" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" width="550" height="400"><param name="movie" value="http://prezi.com/bin/preziloader.swf"/><param name="allowfullscreen" value="true"/><param name="allowscriptaccess" value="always"/><param name="bgcolor" value="#ffffff"/><param name="flashvars" value="prezi_id=uj6xu0za4jti&amp;lock_to_path=1&amp;color=ffffff&amp;autoplay=no&amp;autohide_ctrls=0"/><embed id="preziEmbed_uj6xu0za4jti" name="preziEmbed_uj6xu0za4jti" src="http://prezi.com/bin/preziloader.swf" type="application/x-shockwave-flash" allowfullscreen="true" allowscriptaccess="always" width="550" height="400" bgcolor="#ffffff" flashvars="prezi_id=uj6xu0za4jti&amp;lock_to_path=1&amp;color=ffffff&amp;autoplay=no&amp;autohide_ctrls=0"></embed></object><div class="prezi-player-links"></div></div> </center></html>

Video

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Thank you

<html><a href = 'http://www.biomod.net'><img src= http://openwetware.org/images/0/0c/Biomod_logo.png width=220px> </a></html> <html><a href = 'http://www.wyss.harvard.edu'><img src= http://openwetware.org/images/f/f1/Wyss_logo.gif width=220px> </a></html> <html><a href = 'http://www.hhmi.org'><img src= http://openwetware.org/images/a/ae/Hhmi2.jpg width=220px> </a></html> <html><a href = 'http://www.harvard.edu'><img src= http://openwetware.org/images/9/9c/Harvard_shield_wreath.png width=100px> </a></html>

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