Biomod/2011/Harvard/HarvarDNAnos: Difference between revisions
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==Team Abstract== | |||
Previous work has showcased the use of scaffolded DNA origami to self-assemble nanoscale, three-dimensional shapes with enclosed, hollow interiors. We explore the possibility of using such structures to load, entrap, and release soluble nanoscale cargo, with potential future applications in drug delivery and molecular signal amplification. Specifically, we design a rectangular box structure that can encapsulate gold nanoparticles and subsequently release them by a two-step process: a) photocleavage, which solubilizes the nanoparticle within the box, and b) introduction of a DNA signal, which opens the box by strand-displacement. We further investigate the use of an existing spherical origami design for similar purposes, and demonstrate opening of the sphere. | |||
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<a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos" width="300" height="100" target="_blank" name="The HarvardDNAnos"> | <a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos" width="300" height="100" target="_blank" name="The HarvardDNAnos"> | ||
<img src=http://openwetware.org/images/7/77/Biomod3.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> | <img src=http://openwetware.org/images/7/77/Biomod3.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> | ||
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<img src=http://openwetware.org/images/4/44/Perkonsphoto1.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> | <img src=http://openwetware.org/images/4/44/Perkonsphoto1.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> | ||
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Revision as of 09:00, 15 October 2011
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<html><center><a href='http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos'><img src=http://openwetware.org/images/1/1c/Hdnaheader.jpg width=800px></a></center></html>
Team AbstractPrevious work has showcased the use of scaffolded DNA origami to self-assemble nanoscale, three-dimensional shapes with enclosed, hollow interiors. We explore the possibility of using such structures to load, entrap, and release soluble nanoscale cargo, with potential future applications in drug delivery and molecular signal amplification. Specifically, we design a rectangular box structure that can encapsulate gold nanoparticles and subsequently release them by a two-step process: a) photocleavage, which solubilizes the nanoparticle within the box, and b) introduction of a DNA signal, which opens the box by strand-displacement. We further investigate the use of an existing spherical origami design for similar purposes, and demonstrate opening of the sphere. |
<iframe width="560" height="315" src="http://www.youtube.com/embed/7gjLVt9onsw?rel=0" frameborder="0" allowfullscreen></iframe> </html> |
<a href="http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos" width="300" height="100" target="_blank" name="The HarvardDNAnos"> <img src=http://openwetware.org/images/7/77/Biomod3.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/f/f2/Sphere_Image.jpeg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/c/c3/2011-07-06_Sphereunpurifiedopen_TEM.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/3/36/2011-07-06_Sphereunpurifiedclosed_AFM2.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/4/46/Photo_on_2011-06-24_at_16.37.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/1/1e/Perkons2.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/c/c6/DLS_gold15nm.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/1/16/2011-06-06_figure2.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/e/e8/Photo_on_2011-07-01_at_16.26.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/b/bd/IMG_1270.JPG alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/d/d2/2011-07-06_Sphereunpurifiedopen_AFM2.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/c/c3/2011-07-06_Sphereunpurifiedclosed_TEM.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/2/2f/Photo_on_2011-07-12_at_11.33.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/f/fd/Multiplednaonnp.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/b/b8/Screen_shot_2011-07-07_at_3.36.05_PM.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/d/dd/Screen_Shot_2011-08-04_at_5.22.43_PM.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/0/03/IMG_1272.JPG alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/6/62/Zoominwithpolyt.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/c/ce/Photo_on_7-22-11_at_2.54_PM_-2.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/9/98/Nanoparticle_Chain.png alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/1/17/IMG_1276.JPG alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/3/3e/2011-08-10_spheregelpur8bp_bottom1.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0> <img src=http://openwetware.org/images/4/44/Perkonsphoto1.jpg alt="The HarvardDNAnos" height="96" width="128" border=0><img src=http://t0.gstatic.com/images?q=tbn:ANd9GcS6DTgqwbn8N3MWNZfOs78p0B_21NDZgK4YuOoRRVKCtACSCzsm4g" height="96" width="25" border=0>
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