Biomod/2011/Columbia/MotorProTeam:Results: Difference between revisions
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===Alignment=== | ===Alignment=== | ||
Early experiments focused on using flow to align microtubules. The group performed motility assays, then flowed an antifade solution (a solution that removes unattached microtubules from the surface) containing AMP-PNP as a method of securing the microtubules in their positions for imaging. This method proved unreliable. While flow occasionally aligned a small proportion of the microtubules, in general this procedure did not work. | Early experiments focused on using flow to align microtubules. The group performed motility assays, then flowed an antifade solution (a solution that removes unattached microtubules from the surface) containing AMP-PNP as a method of securing the microtubules in their positions for imaging. This method proved unreliable. While flow occasionally aligned a small proportion of the microtubules, in general this procedure did not work. [[Image:Microtubule_flow_alignment_Columbia_biomod_2011.png|thumb|alt=test|Image of flow test]] | ||
Currently, the team is investigating using a protein coated / blank surface boundary as a method to align microtubules. Microtubules that align themselves into the blank region of the flow cell (held at the positive end by a single kinesin motor protein) do not move out of alignment because the majority of their length is not acted upon by any motor proteins. | Currently, the team is investigating using a protein coated / blank surface boundary as a method to align microtubules. Microtubules that align themselves into the blank region of the flow cell (held at the positive end by a single kinesin motor protein) do not move out of alignment because the majority of their length is not acted upon by any motor proteins. | ||
Revision as of 12:32, 31 May 2011
Alignment
Early experiments focused on using flow to align microtubules. The group performed motility assays, then flowed an antifade solution (a solution that removes unattached microtubules from the surface) containing AMP-PNP as a method of securing the microtubules in their positions for imaging. This method proved unreliable. While flow occasionally aligned a small proportion of the microtubules, in general this procedure did not work.
Currently, the team is investigating using a protein coated / blank surface boundary as a method to align microtubules. Microtubules that align themselves into the blank region of the flow cell (held at the positive end by a single kinesin motor protein) do not move out of alignment because the majority of their length is not acted upon by any motor proteins.
