Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Results

From OpenWetWare
Jump to navigationJump to search

Thursday, April 18, 2024

Home

Members

Project

Protocols

Progress

Discussion

References


Results

While the overall project is the sorting of cargos on origami, we expected verification of all of the mechanisms would take longer than one summer. Therefore, this summer was spent verifying two aspects of the project:

  1. Verifying that the proposed mechanisms work when the strands are free-floating in solution (i.e. not bound to origami).
  2. Verifying that the random walking mechanism works as intended on origami.

Verification of Overall Mechanisms in Solution

Gel Verification

This gel tests the ability of the walker to bind to its tracks and the walker triggering mechanism. Lane 1: W; 2: TR1; 3: TR2; 4: WI; 5: WT; 6: W + TR1 → (W-TR1); 7: W + TR2 → (W-TR2); 8: W + WI → (W-WI); 9: WI + WT → (WI-WT); 10: W + TR1 + WI → (W-TR1-WI); 11: (W-WI-TR1) + WT → (W-TR1) + (WI-WT).

SPEX Verification

Verification of Random Walking Mechanism on Origami

SPEX Verification

AFM Verification