Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Results

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(Gel Verification)
(Gel Verification)
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===Gel Verification===
===Gel Verification===
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This gel tests the ability of the walker to bind to its  
This gel tests the ability of the walker to bind to its  
tracks and the walker triggering mechanism.  
tracks and the walker triggering mechanism.  

Revision as of 18:56, 12 October 2011

Image:DeoxyriboNucleicAwesomeHeader.jpg

Wednesday, October 1, 2014

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Results

While the overall project is the sorting of cargos on origami, we expected verification of all of the mechanisms would take longer than one summer. Therefore, this summer was spent verifying two aspects of the project:

  1. Verifying that the proposed mechanisms work when the strands are free-floating in solution (i.e. not bound to origami).
  2. Verifying that the random walking mechanism works as intended on origami.

Contents


Verification of Overall Mechanisms in Solution

Gel Verification

This gel tests the ability of the walker to bind to its tracks and the walker triggering mechanism. Lane 1: W; 2: TR1; 3: TR2; 4: WI; 5: WT; 6: W + TR1 → (W-TR1); 7: W + TR2 → (W-TR2); 8: W + WI → (W-WI); 9: WI + WT → (WI-WT); 10: W + TR1 + WI → (W-TR1-WI); 11: (W-WI-TR1) + WT → (W-TR1) + (WI-WT). Image:Dnawesome_gel1.png Image:Dnawesome_gel2.png Image:Dnawesome_gel3.png Image:Dnawesome_gel4.png Image:Dnawesome_gel5.png Image:Dnawesome_gel6.png Image:dnawesome_gel7.png

SPEX Verification

Verification of Random Walking Mechanism on Origami

SPEX Verification

AFM Verification

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