Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Protocols/Origami Purification

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(New page: == SPEX== 1. Turn on the lamp and wait for 30 min for the lamp to warm up. 2. Launch the software: Instrument control center 3. Temperature set up Click the third square icon “...)
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== SPEX==
 
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1. Turn on the lamp and wait for 30 min for the lamp to warm up.
 
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2. Launch the software: Instrument control center
 
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3. Temperature set up
 
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Click the third square icon “Visual setup”
 
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Temperature control icon: Turn on and Set to 25 degreeC
 
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Choose T bath (internal).
 
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4. Wash the cuvettes.
 
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Distilled Water (DW) (10 times) 70% ethanol (twice) DW (5 times) 70% ethanol (once)
 
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Air dry the cuvettes for 1 h.
 
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5. Clean the cuvettes using lens paper.
 
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6. Click second square icon “real time display”.
 
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High voltage: HV on, S 950, T 950
 
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Bandpass : 2
 
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7. Click the last icon “run CWA”.
 
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Open the file “ROX_CWA”
 
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Optional mode: kinetics, Integration time: 10s
 
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Define: Repeat acquisition: 1 min (minimum) for a total of ~ min, depending on how long you would like to run the experiment.
 
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8. Mixing: Mix the sample with 1X TE/Mg++ buffer inside cuvettes by pipetting for 30 times.
 
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9. Start acquisition. Run sample.
 
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The data should not exceed 1 million (1000,000).
 
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10. To add more samples (for example, walker triggers) in the middle of SPEX experiment
 
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When the status is “waiting”, click “stop”.
 
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Add samples and mix. Replace cuvettes into the SPEX machine.
 
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11. Save the data
 
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When the status is “waiting”, click “stop”.
 
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Click “yes”
 
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Save data in two forms (DWA datafile, txt file)
 
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Check if the data is properly saved.
 
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*If you want to see the reference signals, go to options: view option and display raw R.
 

Revision as of 22:51, 2 November 2011

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