Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Protocols: Difference between revisions
YAE LIM Lee (talk | contribs) |
YAE LIM Lee (talk | contribs) No edit summary |
||
Line 10: | Line 10: | ||
*How we ran experiments... | *How we ran experiments... | ||
Revision as of 15:36, 12 October 2011
Friday, April 26, 2024
|
ProtocolsThings that need to be written
SPEX protocol
Bottom big on/off button is for the machine. It is always on. Top small one is for the lamp. If there’s somebody who will use the SPEX tomorrow, then do not turn off the lamp after the experiment.
Default: Click OK
Click the third square icon called “Visual setup” Click the middle square Temperature control icon: Turn on and Set to 25 degreeC Choose T bath (internal). NOT Probe (external).
Be careful! Hold only top or bottom. DW (10 times) 70% ethanol (twice) DW (5 times) 70% ethanol (once) While you wash, you can touch the side. Hold it in a stable way. Let it dry (upside down)
High voltage: HV on, S 950, T 950 Bandpass : Set 2 for everything
Open a file called “ROX_CWA” from e:\SPEX DATA\biomod Optional mode: kinetics, Integration time: 10s Define: Repeat acquisition: 1 min (minimum) for total of 180 min (3 hours)
Range of the real data should not exceed 1 million (1000,000) Number we see is S/R will be (100 million)
When the status is “waiting”, click “stop”. Don’t click anything else. Add and mix. After you put the cuvettes inside the box, click “no”.
When the status is “waiting”, click “stop”. Click “yes” Save data in two forms (DWA datafile, multi~ test) Check if the data is properly saved: Check the last data.
How to Open SPEX Data in MATLAB
|